Certificate of Analysis: Live Reef Sand, Batch LRS G10.11
Certificate of Analysis
lrs.g10.11_Certificate_of_Analysis
About this report
This report summarizes the results from DNA analysis of the community growing in a batch of Live Reef Sand. Universal genetic markers were amplified from microbial (prokaryotic) and other (eukaryotic) DNA in the sample, and thousands of individual DNA molecules from this mixture were sequenced. Each sequence was then compared with public DNA databases to identify its origin. This report summarizes the kinds of organisms detected in the sample, with a focus on beneficial microbes, pathogens, and parasites.
Information about this sample
| Sample ID | lrs.g10.11 |
| Batch Name | G08-04-2025 |
| Sample Date | Oct 12, 2025 10:15 AM |
Microbial Diversity
This sample contained DNA from 587 different types of microbes.
This pie chart shows the diversity of each microbial family found in the sample. The size of each slice is proportional to the number of different types (labels on each slice). For clarity, only families with 2 or more types are shown here. Unidentified sequences lacking any match in public databases are not shown.
Nitrifying Community
Ammonia-Oxidizing Microbes
| Type | Percent of Community | Genus | Species |
|---|---|---|---|
| 73 | 2.26 | Nitrosopumilus | Unknown |
| 760 | 0.78 | Nitrosopumilus | Unknown |
| 14173 | 0.45 | Nitrosopumilus | Unknown |
| 20 | 0.29 | Nitrosopumilus | Unknown |
| 640 | 0.16 | Nitrosopumilus | Unknown |
Nitrite-Oxidizing Bacteria
| Type | Percent of Community | Genus | Species |
|---|---|---|---|
| 218731 | 0.07 | Nitrospina | Unknown |
| 3394 | 0.06 | Nitrospina | Unknown |
The nitrifying community in this sample included 7 different types of microbes.
Bacterial Pathogens
None found.
Complete list of pathogens we screened for:
Eubacterium tarantellae, Lactococcus garvieae, Enterococcus seriolicida, Streptococcus parauberis, Streptococcus iniae, Mycobacterium chelonei, Mycobacterium fortuitum, Mycobacterium marinum, Mycobacterium neoaurum, Nocardia asteroides, Nocardia salmonicida, Nocardia seriolae, Renibacterium salmoninarum, Aeromonas jandaei, Aeromonas salmonicida, Serratia liquefaciens, Chryseobacterium balustinum, Chryseobacterium scophthalmum, Tenacibaculum maritimus, Tenacibaculum ovolyticus, Pasteurella skyensis, Pseudomonas anguilliseptica, Moritella marina, Moritella viscosa, Photobacterium damselae, Shewanella putrefaciens, Vibrio alginolyticus, Vibrio cholerae, Vibrio fischeri, Vibrio furnissii, Vibrio harveyi, Vibrio carchariae, Vibrio trachuri, Vibrio ichthyoenteri, Vibrio logei, Vibrio ordalii, Vibrio pelagius, Vibrio salmonicida, Vibrio splendidus, Halomonas cupida, Piscirickettsia salmonis, Vibrio shiloi, Vibrio coralliilyticus, Vibrio harveyi, Aurantimonas coralicida, Vibrio rotiferianus, Vibrio alginolyticus, Vibrio proteolyticus, Vibrio charcharvina, Serratia marscesens, Aquarickettsia rohweri
Eukaryotic Biodiversity
This sample contained DNA from 422 different Eukaryotic sources.
This pie chart shows the diversity of each Eukaryotic Phylum detected in the sample. The size of each slice is proportional to the number of different types from each phylum. For clarity, only families with 3 or more types are shown here. Unidentified sequences lacking any match in public databases are not shown.
Parasites
None of the DNA sequences from this sample matched known parasites.
| Group | Description | Percent of eDNA |
|---|---|---|
| Amyloodinium | Dinoflagellate parasites responsible for the fish disease Marine Velvet | 0 |
| Cryptocaryon | Ciliate parasites responsible for the fish disease Ich | 0 |
| Brooklynella | Ciliate parasites responsible for the fish disease Brook | 0 |
| Uronema | Ciliate parasites responsible for a fish disease called by the same name | 0 |
| Phestilla | Montipora-eating Nudibranchs | 0 |
| Amakusaplana | Acropora-eating Flatworms | 0 |
| Ancyrocephalidae | Flatworms often called Flukes that parasitize fish | 0 |
| Gyrodactylidae | Flatworms often called Flukes that parasitize fish | 0 |
| Capsalidae | Flatworms often called Flukes that parasitize fish | 0 |
| Turbonilla | Snails in the family Pyramidellidae that parasitize giant clams | 0 |
| Trichodina | A ciliated protozoan parasite of fish | 0 |
Other Animal Pests
None of the DNA sequences from this sample matched known animal pests.
| Group | Description | Percent of eDNA |
|---|---|---|
| Aiptasia | The glass anemone, a ubiquitous pest | 0 |
| Thylacodes | Vermetid snails | 0 |
| Asterina | A group of sea stars that includes coral predators | 0 |
Complete list of Eukaryotes identified
This list includes all Eukaryotes (Animals, Protists, and anything else besides Bacteria or Archaea) that were detected in the sample and identified based on comparison with public DNA sequence databases. DNA sequences that lacked any matches in public datases could not be identified, and are not shown here. The list is sorted by abundance, from highest to lowest.
Coquimba ishizakii (Arthropoda), Bradyagaue sp. (Arthropoda), Robustaurila salebrosa (Arthropoda), Howeina sp. (Arthropoda), Xyrodromeus namarona (Arthropoda), Acarothrix sp. (Arthropoda), Simognathus sp. (Arthropoda), Microcaeculus sp. (Arthropoda), Halacarellus sp. (Arthropoda), Membranipora sp. (Arthropoda), Copidognathus sp. (Arthropoda), uncultured eukaryote 495827 (Ciliophora), Euplotes orientalis (Ciliophora), Glauconema trihymene (Ciliophora), Cothurnia salina (Ciliophora), Pseudokeronopsis carnea (Ciliophora), Eutintinnus cf. (Ciliophora), Pseudokeronopsis erythrina (Ciliophora), uncultured eukaryote 515761 (Ciliophora), uncultured marine 509090 (Diatomea), Rhaphoneis belgicae (Diatomea), Pauliella sp. (Diatomea), Psammodictyon constrictum (Diatomea), Pinnularia substreptoraphe (Diatomea), Pseudohimantidium pacificum (Diatomea), uncultured marine 511875 (Diatomea), uncultured marine 511894 (Diatomea), uncultured marine 510447 (Diatomea), Nitzschia sigma (Diatomea), uncultured marine 515394 (Diatomea), uncultured marine 515449 (Diatomea), Rhaphoneis sp. (Diatomea), Nitzschia longissima (Diatomea), Monosiga brevicollis (Eukaryota), Paraphysomonas imperforata (Eukaryota), Choanoeca perplexa (Eukaryota), uncultured marine 1786 (Eukaryota), Symbiochloris sp. (Eukaryota), Ulvophyceae sp. (Eukaryota), uncultured eukaryote 497070 (Eukaryota), eukaryotic picoplankton (Eukaryota), uncultured marine 510577 (Eukaryota), metagenome (Eukaryota), Paraphysomonas bandaiensis (Eukaryota), uncultured marine 515420 (Eukaryota), Paraphysomonas sp. (Eukaryota), uncultured eukaryote 515423 (Eukaryota), uncultured stramenopile 515425 (Eukaryota), uncultured eukaryote 515428 (Eukaryota), Hartaetosiga gracilis (Eukaryota), uncultured Corallinales 509248 (Florideophycidae), uncultured Corallinales 509705 (Florideophycidae), uncultured Corallinales 515553 (Florideophycidae), Aspidiophorus polystictos (Gastrotricha), Thraustochytriidae sp. (Labyrinthulomycetes), uncultured marine 509695 (Labyrinthulomycetes), uncultured marine 510033 (Labyrinthulomycetes), uncultured marine 510592 (Labyrinthulomycetes), uncultured marine 511885 (Labyrinthulomycetes), uncultured eukaryote 511886 (Labyrinthulomycetes)
Complete List of Microbes Identified
This list includes all microbes (Bacteria & Archae) that were detected in the sample and identified based on comparison with public DNA sequence databases. DNA sequences that lacked any matches in public datases could not be identified, and are not shown here. The list is sorted by abundance, from highest to lowest.
Unclassified Legionellaceae 117161, BD2-13 sp., Unclassified Coxiellaceae 976, Nitrosopumilus sp., Unclassified Hyphomicrobiaceae 1489, Mycobacterium sp., Unclassified Legionellaceae 251934, Microbulbifer sp., Unclassified Bacteria 152381, Unclassified Bacteria 2368, Photobacterium sp., Unclassified Rhodobacteraceae 276, Unclassified Bacteria 6811, Unclassified Hyphomicrobiaceae 273, Unclassified Bacteria 11039, Unclassified Bacteria 336000, Unclassified Bacteria 10241, Bdellovibrio sp., Unclassified Bacteria 1394, Unclassified Pirellulaceae 977, Unclassified Hyphomicrobiaceae 10456, Unclassified Alteromonadaceae 336030, Unclassified Bacteria 467, Unclassified Hyphomicrobiaceae 85007, Unclassified Hyphomicrobiaceae 286, Unclassified Bacteria 26819, Unclassified Bacteria 122847, Unclassified Hyphomicrobiaceae 955, Unclassified Bacteria 1447, Unclassified Rhodospirillaceae 235424, Unclassified Alteromonadaceae 88, Unclassified Bacteria 5367, Unclassified Erythrobacteraceae 13907, Unclassified Simkaniaceae 797, Unclassified Bacteria 14595, Unclassified Kordiimonadaceae 336106, Unclassified Flavobacteriaceae 1198, Ucs1325 sp., Glaciecola sp., Photobacterium angustum, Oleibacter sp., Unclassified Bacteria 336144, Unclassified Bacteriovoracaceae 12300, Unclassified Bacteriovoracaceae 34824, Unclassified Bacteria 68084, Clostridium sp., Unclassified Pirellulaceae 26156, Unclassified Bacteria 336182, Balneola sp., Unclassified Bacteria 4384, Unclassified Bacteria 27456, Unclassified Bacteriovoracaceae 67724, Unclassified Bacteria 105166, Unclassified Hyphomonadaceae 817, Unclassified Saprospiraceae 6824, Unclassified Bacteria 107030, Unclassified Alteromonadaceae 14898, Unclassified Bacteria 28067, Unclassified Bacteria 265885, Unclassified Bacteria 336380, Unclassified Methylophilaceae 392, Unclassified Phyllobacteriaceae 704, Unclassified Bacteria 3423, Unclassified Bacteria 12849, Unclassified Kordiimonadaceae 26209, Unclassified Piscirickettsiaceae 167734, Nitrospina sp., Unclassified Bacteria 949, Planctomyces sp., Coraliomargarita sp., Unclassified Pirellulaceae 11232, Unclassified Bacteria 11765, Unclassified Bacteria 107029, Unclassified Bacteria 133151, Unclassified Kordiimonadaceae 220101, Unclassified NS11-12 318639, Verrucomicrobium sp., Unclassified Bacteria 336473, Unclassified Bacteria 6805, Unclassified Bacteria 336537, Unclassified Thiotrichaceae 336538, Unclassified Alteromonadaceae 585, Unclassified Bacteria 25624, Unclassified Bacteria 68414, Unclassified Cyanobacteriaceae 258835, Unclassified Pirellulaceae 336633, Unclassified Desulfovibrionaceae 96, Unclassified Bacteria 1141, Unclassified Bacteria 67898, Unclassified Bacteria 108985, Candidatus sp., Marinicella sp., Unclassified [Amoebophilaceae] 16688, Salinisphaera sp., Unclassified Bacteria 319001, Unclassified Verrucomicrobiaceae 336498, Unclassified Bacteria 336900, Francisella sp., Unclassified Rhodospirillaceae 982, Unclassified Oceanospirillaceae 37511, Unclassified Bacteria 235564, Unclassified Bacteria 336376, Unclassified Pirellulaceae 337087, Unclassified Kordiimonadaceae 337092, Unclassified Bacteria 337093, Unclassified Bacteria 904, Plesiocystis sp., Sulfurimonas sp., Unclassified Coxiellaceae 337313, Unclassified Bacteria 337325, Unclassified Pirellulaceae 337329, Maricaulis sp., Unclassified Lentisphaeraceae 1012, Arcobacter sp., Unclassified Ellin6075 28540, Unclassified Bacteria 191554, Unclassified Pirellulaceae 201307, Unclassified Alteromonadaceae 248093, Coxiella sp., Unclassified Xenococcaceae 337627, Unclassified Bacteria 337628, Unclassified Rhodobacteraceae 50575, Mycoplasma sp., Unclassified Bacteria 337985, Unclassified wb1_P06 338008, Unclassified Piscirickettsiaceae 338009, Unclassified Pirellulaceae 338010, Unclassified Bacteria 338021, Unclassified Bacteria 338023, Unclassified Bacteria 141, Deinococcus sp., Unclassified Bacteria 13895, Bacillus sp., Unclassified Cryomorphaceae 52791, Unclassified Alteromonadaceae 229824, Unclassified Rhodospirillaceae 338485, Unclassified OM60 338486, Unclassified HTCC2188 338489, Streptococcus sp., Unclassified Rhodospirillaceae 337323, Unclassified Pirellulaceae 339000, Unclassified Alteromonadaceae 200038, Unclassified Hyphomicrobiaceae 304640, HB2-32-21 sp., Anaerospora sp.
DNA analysis conducted by AquaBiomics LLC.