Certificate of Analysis: Live Reef Rubble, Batch LRR G11.1
Certificate of Analysis
lrr.g11.1_Certificate_of_Analysis
About this report
This report summarizes the results from DNA analysis of the community growing in and on a batch of Live Reef Rubble. Universal genetic markers were amplified from microbial (prokaryotic) and other (eukaryotic) DNA in the sample, and thousands of individual DNA molecules from this mixture were sequenced. Each sequence was then compared with public DNA databases to identify its origin. This report summarizes the kinds of organisms detected in the sample, with a focus on beneficial microbes, pathogens, and parasites.
Information about this sample
| Sample ID | lrr.g11.1 |
| Batch Name | g12-15-25 |
| Sample Date | 1/16/26 10:00 AM |
Microbial Diversity
This sample contained DNA from 475 different types of microbes.
This pie chart shows the diversity of each microbial family found in the sample. The size of each slice is proportional to the number of different types (labels on each slice). For clarity, only families containing 2 or more types are shown here. Unidentified sequences lacking any match in public databases are not shown.
Nitrifying Community
Ammonia-Oxidizing Microbes
| Type | Percent of Community | Genus | Species |
|---|---|---|---|
| 58445 | 0.35 | Unknown | Unknown |
| Type | Percent of Community | Genus | Species |
|---|---|---|---|
| 73 | 0.91 | Nitrosopumilus | Unknown |
| 29969 | 0.62 | Unknown | Unknown |
| 2996 | 0.19 | Nitrosopumilus | Unknown |
| 248127 | 0.18 | Unknown | Unknown |
| 74696 | 0.11 | Cenarchaeum | symbiosum |
Nitrite-Oxidizing Bacteria
| Type | Percent of Community | Genus | Species |
|---|---|---|---|
| 9977 | 0.01 | Unknown | Unknown |
The nitrifying community in this sample included 7 different types of microbes.
Bacterial Pathogens
None found.
Complete list of pathogens we screened for:
Eubacterium tarantellae, Lactococcus garvieae, Enterococcus seriolicida, Streptococcus parauberis, Streptococcus iniae, Mycobacterium chelonei, Mycobacterium fortuitum, Mycobacterium marinum, Mycobacterium neoaurum, Nocardia asteroides, Nocardia salmonicida, Nocardia seriolae, Renibacterium salmoninarum, Aeromonas jandaei, Aeromonas salmonicida, Serratia liquefaciens, Chryseobacterium balustinum, Chryseobacterium scophthalmum, Tenacibaculum maritimus, Tenacibaculum ovolyticus, Pasteurella skyensis, Pseudomonas anguilliseptica, Moritella marina, Moritella viscosa, Photobacterium damselae, Shewanella putrefaciens, Vibrio alginolyticus, Vibrio cholerae, Vibrio fischeri, Vibrio furnissii, Vibrio harveyi, Vibrio carchariae, Vibrio trachuri, Vibrio ichthyoenteri, Vibrio logei, Vibrio ordalii, Vibrio pelagius, Vibrio salmonicida, Vibrio splendidus, Halomonas cupida, Piscirickettsia salmonis, Vibrio shiloi, Vibrio coralliilyticus, Vibrio harveyi, Aurantimonas coralicida, Vibrio rotiferianus, Vibrio alginolyticus, Vibrio proteolyticus, Vibrio charcharvina, Serratia marcescens, Aquarickettsia rohweri
Eukaryotic Biodiversity
This sample contained DNA from 720 different Eukaryotic sources.
This pie chart shows the diversity of each Eukaryotic Phylum detected in the sample. The size of each slice is proportional to the number of different types from each phylum. For clarity, only families containing 2 or more types are shown here. Unidentified sequences lacking any match in public databases are not shown.
Parasites
None of the DNA sequences from this sample matched known parasites.
| Group | Description | Percent of eDNA |
|---|---|---|
| Amyloodinium | Dinoflagellate parasites responsible for the fish disease Marine Velvet | 0 |
| Cryptocaryon | Ciliate parasites responsible for the fish disease Ich | 0 |
| Brooklynella | Ciliate parasites responsible for the fish disease Brook | 0 |
| Uronema | Ciliate parasites responsible for a fish disease called by the same name | 0 |
| Phestilla | Montipora-eating Nudibranchs | 0 |
| Amakusaplana | Acropora-eating Flatworms | 0 |
| Ancyrocephalidae | Flatworms often called Flukes that parasitize fish | 0 |
| Gyrodactylidae | Flatworms often called Flukes that parasitize fish | 0 |
| Capsalidae | Flatworms often called Flukes that parasitize fish | 0 |
| Turbonilla | Snails in the family Pyramidellidae that parasitize giant clams | 0 |
| Trichodina | A ciliated protozoan parasite of fish | 0 |
Other Animal Pests
None of the DNA sequences from this sample matched known animal pests.
| Group | Description | Percent of eDNA |
|---|---|---|
| Aiptasia | The glass anemone, a ubiquitous pest | 0 |
| Thylacodes | Vermetid snails | 0 |
| Asterina | A group of sea stars that includes coral predators | 0 |
Complete list of Eukaryotes identified
This list includes all Eukaryotes (Animals, Protists, and anything else besides Bacteria or Archaea) that were detected in the sample and identified based on comparison with public DNA sequence databases. DNA sequences that lacked any matches in public datases could not be identified, and are not shown here. The list is sorted by abundance, from highest to lowest.
Chitinopoma serrula (Annelida), Novafabricia sp. (Annelida), uncultured eukaryote 541720 (Apicomplexa), Lecudina phyllochaetopteri (Apicomplexa), Sellea sp. (Arthropoda), Saccharum hybrid (Arthropoda), Artemia salina (Arthropoda), uncultured eukaryote 542594 (Arthropoda), Pseudanaphothrips achaetus (Arthropoda), Aspergillus cristatus (Ascomycota), Stagonosporopsis cucurbitacearum (Ascomycota), Humulus lupulus (Ascomycota), Graminopassalora graminis (Ascomycota), Aspergillus amstelodami (Ascomycota), Wallemia sebi (Basidiomycota), Postia placenta (Basidiomycota), Psathyrella candolleana (Basidiomycota), Cryptococcus sp. (Basidiomycota), Filobasidium floriforme (Basidiomycota), Goffeauzyma gastrica (Basidiomycota), uncultured eukaryote 1396 (Bicosoecida), Licmophora sp. (Bicosoecida), metagenome (Centrohelida), Paradinium poucheti (Cercozoa), uncultured marine 15485 (Cercozoa), Gromia oviformis (Cercozoa), uncultured eukaryote 2529 (Cercozoa), Protaspis grandis (Cercozoa), Reckertia gemma (Cercozoa), uncultured Cercozoa 54890 (Cercozoa), Protaspis sp. (Cercozoa), uncultured marine 60804 (Cercozoa), Ventrifissura sp. (Cercozoa), marine metagenome (Cercozoa), uncultured eukaryote 541785 (Cercozoa), uncultured marine 541812 (Cercozoa), Vampyrellida sp. (Cercozoa), Ventrifissura artocarpoidea (Cercozoa), uncultured marine 425196 (Cercozoa), uncultured Cercozoa 542384 (Cercozoa), metagenome (Cercozoa), Metanophrys sinensis (Ciliophora), Moneuplotes minuta (Ciliophora), Heterohartmannula fangi (Ciliophora), Loxophyllum shini (Ciliophora), Paracineta limbata (Ciliophora), Euplotes euryhalinus (Ciliophora), Diophrys oligothrix (Ciliophora), Holosticha diademata (Ciliophora), Stichotricha aculeata (Ciliophora), Euplotes rariseta (Ciliophora), Holosticha heterofoissneri (Ciliophora), Glauconema trihymene (Ciliophora), Diophrys parappendiculata (Ciliophora), Pseudovorticella paracratera (Ciliophora), uncultured alveolate 60809 (Ciliophora), uncultured eukaryote 542028 (Ciliophora), Stichotrichia sp. (Ciliophora), Amphileptus spiculatus (Ciliophora), Euplotes sp. (Ciliophora), Vorticella oceanica (Ciliophora), Aristerostoma sp. (Ciliophora), Dysteria pectinata (Ciliophora), uncultured ciliate 2052 (Ciliophora), Amphileptus litonotiformis (Ciliophora), Protogastrostyla pulchra (Ciliophora), uncultured ciliate 245931 (Ciliophora), Varistrombidium kielum (Ciliophora), Thigmokeronopsis sp. (Ciliophora), Phyllangia mouchezii (Cnidaria), Astrangia poculata (Cnidaria), Xenia sp. (Cnidaria), Mycetophyllia aliciae (Cnidaria), uncultured eukaryote 6222 (Diatomea), Halamphora subtropica (Diatomea), uncultured marine 472638 (Diatomea), uncultured marine 541685 (Diatomea), Psammodictyon constrictum (Diatomea), Pleurosigma sp. (Diatomea), uncultured marine 541667 (Diatomea), Entomoneis ornata (Diatomea), uncultured marine 541909 (Diatomea), Licmophora juergensii (Diatomea), uncultured marine 166879 (Diatomea), Licmophora sp. (Diatomea), Convoluta convoluta (Diatomea), Craticula pseudocitrus (Diatomea), Sellaphora sp. (Diatomea), Haslea spicula (Diatomea), uncultured marine 152470 (Diatomea), Cocconeis placentula (Diatomea), Phaeodactylum tricornutum (Diatomea), Pfiesteria-like sp. (Dinoflagellata), Symbiodinium sp. (Dinoflagellata), Symbiodinium microadriaticum (Dinoflagellata), Ostreopsis cf. (Dinoflagellata), uncultured eukaryote 50575 (Dinoflagellata), uncultured eukaryote 407479 (Dinoflagellata), Amphidinium sp. (Dinoflagellata), Prorocentrum arenarium (Dinoflagellata), uncultured eukaryote 203436 (Dinoflagellata), uncultured eukaryote 295341 (Dinoflagellata), Symbiodinium minutum (Dinoflagellata), uncultured eukaryote 542387 (Dinoflagellata), Dinophyta sp. (Dinoflagellata), Diplonema sp. (Euglenozoa), uncultured eukaryote 17870 (Euglenozoa), Diplonemida sp. (Euglenozoa), Hemistasiidae sp. (Euglenozoa), Notosolenus urceolatus (Euglenozoa), Neobodo designis (Euglenozoa), uncultured kinetoplastid 13113 (Euglenozoa), uncultured eukaryote 541798 (Euglenozoa), uncultured eukaryote 542293 (Euglenozoa), Klosteria bodomorphis (Euglenozoa), Kinetoplastida sp. (Euglenozoa), uncultured eukaryote 291943 (Euglenozoa), Rhynchopus sp. (Euglenozoa), uncultured eukaryote 32619 (Eukaryota), Paraphysomonas imperforata (Eukaryota), uncultured eukaryote 541736 (Eukaryota), Paramoeba branchiphila (Eukaryota), eukaryote clone (Eukaryota), uncultured eukaryote 1494 (Eukaryota), Ulvophyceae sp. (Eukaryota), Ciliophrys infusionum (Eukaryota), Vannella sp. (Eukaryota), uncultured eukaryote 541928 (Eukaryota), Rhodomonas abbreviata (Eukaryota), uncultured eukaryote 541866 (Eukaryota), Amphibiocystidium ranae (Eukaryota), Picochlorum eukaryotum (Eukaryota), uncultured stramenopile 542091 (Eukaryota), Scytosiphon lomentaria (Eukaryota), Anurofeca richardsi (Eukaryota), uncultured eukaryote 380705 (Eukaryota), uncultured eukaryote 541893 (Eukaryota), uncultured stramenopile 541927 (Eukaryota), Carinoma tremaphoros (Eukaryota), uncultured eukaryote 542380 (Eukaryota), uncultured eukaryote 542442 (Eukaryota), uncultured eukaryote 542591 (Eukaryota), uncultured eukaryote 542610 (Eukaryota), Rhodomonas marina (Eukaryota), Rhinomonas pauca (Eukaryota), Rhodomonas sp. (Eukaryota), Anurofeca sp. (Eukaryota), metagenome (Eukaryota), uncultured eukaryote 542906 (Eukaryota), Tetraselmis sp. (Eukaryota), uncultured stramenopile 542919 (Eukaryota), uncultured eukaryote 542986 (Eukaryota), uncultured marine 542987 (Eukaryota), Peyssonnelia novae-hollandiae (Florideophycidae), uncultured Corallinales 32421 (Florideophycidae), uncultured Corallinales 21317 (Florideophycidae), uncultured Corallinales 152450 (Florideophycidae), Peyssonnelia capensis (Florideophycidae), uncultured Corallinales 363979 (Florideophycidae), uncultured eukaryote 541841 (Florideophycidae), Melobesioideae sp. (Florideophycidae), Peyssonnelia sp. (Florideophycidae), uncultured Corallinales 1433 (Florideophycidae), Rhodopeltis borealis (Florideophycidae), uncultured eukaryote 542438 (Florideophycidae), Lithophyllum pustulatum (Florideophycidae), uncultured Corallinales 32614 (Florideophycidae), Cordylecladia erecta (Florideophycidae), uncultured Corallinales 542175 (Florideophycidae), uncultured Corallinales 542633 (Florideophycidae), Oblongichytrium sp. (Labyrinthulomycetes), uncultured eukaryote 72 (Labyrinthulomycetes), Schizochytrium minutum (Labyrinthulomycetes), Thraustochytrium multirudimentale (Labyrinthulomycetes), Thraustochytrium pachydermum (Labyrinthulomycetes), Labyrinthuloides minuta (Labyrinthulomycetes), uncultured eukaryote 4861 (Labyrinthulomycetes), Stellarchytrium dubum (Labyrinthulomycetes), Thraustochytriidae sp. (Labyrinthulomycetes), uncultured stramenopile 21358 (Labyrinthulomycetes), uncultured eukaryote 47361 (Labyrinthulomycetes), uncultured eukaryote 67219 (Labyrinthulomycetes), uncultured eukaryote 1117 (Labyrinthulomycetes), Thraustochytrium sp. (Labyrinthulomycetes), uncultured marine 1339 (Labyrinthulomycetes), uncultured eukaryote 402669 (Labyrinthulomycetes), uncultured eukaryote 541985 (Labyrinthulomycetes), uncultured eukaryote 4141 (Labyrinthulomycetes), uncultured eukaryote 5115 (Labyrinthulomycetes), uncultured eukaryote 408039 (Labyrinthulomycetes), Ulkenia profunda (Labyrinthulomycetes), Haliphthoros sp. (Peronosporomycetes), Olpidiopsis sp. (Peronosporomycetes), Halodaphnea panulirata (Peronosporomycetes), uncultured stramenopile 542619 (Peronosporomycetes), Triticum aestivum (Phragmoplastophyta), Manilkara zapota (Phragmoplastophyta), Spirastrella sp. (Porifera), Leuconia nivea (Porifera), Leucetta sp. (Porifera), Grantiopsis heroni (Porifera), Demospongiae sp. (Porifera), Clathrina luteoculcitella (Porifera), Aphroceras sp. (Porifera), Eilhardia schulzei (Porifera), Spheciospongia vesparium (Porifera), Clathrina sp. (Porifera), Ernstia adusta (Porifera), Leucaltis clathria (Porifera), Murrayona phanolepis (Porifera), Soleneiscus stolonifer (Porifera), uncultured eukaryote 135208 (Protalveolata), uncultured eukaryote 32469 (Protalveolata), uncultured marine 268951 (Protalveolata), uncultured eukaryote 541706 (Protalveolata), uncultured eukaryote 66827 (Protalveolata), uncultured eukaryote 52921 (Protalveolata), uncultured eukaryote 542285 (Protalveolata), uncultured eukaryote 542615 (Protalveolata), uncultured eukaryote 1004 (uncultured), uncultured eukaryote 1059 (uncultured), uncultured eukaryote 542092 (uncultured), uncultured eukaryote 542637 (uncultured)
Complete List of Microbes Identified
This list includes all microbes (Bacteria & Archae) that were detected in the sample and identified based on comparison with public DNA sequence databases. DNA sequences that lacked any matches in public datases could not be identified, and are not shown here. The list is sorted by abundance, from highest to lowest.
Unclassified Desulfovibrionaceae 96, Photobacterium angustum, Unclassified Coxiellaceae 2257, Unclassified Hyphomicrobiaceae 1489, Curtobacterium sp., Clostridium sp., Unclassified Marinilabiaceae 358310, Unclassified Bacteria 171772, Microbulbifer sp., Nitrosopumilus sp., Unclassified Hyphomicrobiaceae 273, Staphylococcus aureus, Unclassified Oleiphilaceae 67653, Unclassified Bacteria 195182, Unclassified Bacteria 9721, Arcobacter sp., Unclassified Bacteria 11774, Unclassified Bacteria 67693, Unclassified Cenarchaeaceae 29969, Unclassified Bacteria 58502, Crocinitomix sp., Desulfofrigus sp., Rhodovulum sp., Unclassified Alteromonadaceae 25663, Unclassified Kordiimonadaceae 336106, Unclassified [Amoebophilaceae] 1322, Unclassified Micrococcaceae 154917, Pseudoalteromonas sp., Unclassified Alteromonadaceae 16245, Unclassified Lentisphaeraceae 15113, Unclassified Phyllobacteriaceae 9876, Unclassified Lentisphaeraceae 1218, Olleya sp., Hirschia baltica, Unclassified Bacteria 233516, Unclassified Rhodobacteraceae 41, Unclassified Nitrosomonadaceae 58445, Unclassified Bacteria 62060, Unclassified Bacteria 10172, Unclassified Vibrionaceae 166, Candidatus sp., Unclassified Bacteria 128, Unclassified Hyphomicrobiaceae 104, Unclassified Hyphomicrobiaceae 3790, Unclassified Vibrionaceae 58111, Unclassified Flavobacteriaceae 328, HTCC sp., Unclassified Legionellaceae 11183, Mycobacterium sp., BD2-13 sp., Unclassified Bacteria 30, Photobacterium rosenbergii, Unclassified Bacteria 994, Pelagicoccus sp., Unclassified Colwelliaceae 164283, Unclassified Alteromonadaceae 358342, Unclassified Bacteria 319159, Unclassified Alteromonadaceae 10024, Unclassified Bacteria 4091, Bdellovibrio sp., Unclassified Pirellulaceae 109572, Amphritea sp., Unclassified Bacteria 106413, Unclassified Bacteria 358323, Unclassified Coxiellaceae 66758, Unclassified Bacteria 358354, Unclassified Colwelliaceae 102236, Unclassified Rhodospirillaceae 122953, Unclassified Coxiellaceae 358361, Unclassified Bacteria 358362, Desulfovibrio sp., Planctomyces sp., Unclassified Victivallaceae 358364, Tenacibaculum sp., Unclassified Phyllobacteriaceae 1520, Unclassified Oleiphilaceae 2222, Unclassified Colwelliaceae 50492, Oleibacter sp., Unclassified Cenarchaeaceae 248127, Unclassified Legionellaceae 309248, Unclassified Clostridiaceae 358372, Unclassified Pelagibacteraceae 1, Unclassified Bacteriovoracaceae 358374, Caldanaerocella sp., Unclassified Colwelliaceae 358382, Propionigenium sp., Francisella sp., Hyphomicrobium zavarzinii, Coraliomargarita sp., Desulfococcus sp., Clostridiisalibacter sp., Unclassified Methylophilaceae 392, Plesiocystis sp., Unclassified Bacteria 18392, Unclassified Saprospiraceae 46810, Kocuria sp., Hyphomicrobium sp., Halomonas sp., Unclassified Hyphomicrobiaceae 9813, Unclassified Bacteria 949, Unclassified Hyphomicrobiaceae 2139, Unclassified Bacteria 2152, Unclassified Rhodospirillaceae 56010, Fusibacter sp., Unclassified Pirellulaceae 977, Unclassified Oceanospirillaceae 24958, Unclassified Bacteria 81096, Unclassified Bacteria 340180, Unclassified Bacteria 4793, Unclassified Bacteria 5367, Unclassified Saprospiraceae 6824, Devosia sp., Unclassified Bacteria 213686, Unclassified Bacteria 36756, Cenarchaeum symbiosum, Unclassified Pirellulaceae 137577, Unclassified JTB36 186605, Unclassified Rhodospirillaceae 27702, Tepidibacter sp., Unclassified Oleiphilaceae 358415, Unclassified Flavobacteriaceae 358428, Unclassified Bacteria 345, Ucs1325 sp., Unclassified [Marinicellaceae] 31880, Unclassified Bacteria 235576, Spirochaeta sp., Unclassified Bacteria 2629, Unclassified Bacteria 6853, Thalassospira sp., Neptunomonas sp., Inquilinus sp., Unclassified Bacteria 190350, Unclassified [Amoebophilaceae] 358451, Cobetia sp., Unclassified Flavobacteriaceae 58290, Glaciecola sp., Unclassified Chitinophagaceae 1434, Unclassified Bacteria 2492, Polaribacter sp., Unclassified Bacteria 72622, Sulfurimonas sp., Unclassified Hyphomonadaceae 290, Methylobacterium sp., Unclassified Bacteria 4372, Unclassified Bacteria 68755, Unclassified Bacteria 84524, Unclassified Hyphomonadaceae 112803, Unclassified Pirellulaceae 358534, Unclassified Lachnospiraceae 358537, Unclassified Bacteria 1347, Finegoldia sp., Unclassified Caulobacteraceae 5440, Aquarickettsia rohweri, Unclassified Bacteria 27890, Unclassified NS11-12 76843, Unclassified Bacteria 358558, Unclassified Bacteria 358560, Unclassified Bacteria 358561, Unclassified Oceanospirillaceae 1096, Agarivorans sp., Unclassified Alteromonadaceae 358600, Achromobacter sp., Unclassified Bacteria 4991, Unclassified Bacteria 7594, Unclassified Bacteria 129144, Unclassified Bacteria 358629, Unclassified Alteromonadaceae 358630, Unclassified Oleiphilaceae 358632, Unclassified Alteromonadaceae 358633, Unclassified [Amoebophilaceae] 1274, Unclassified Oleiphilaceae 139262, Unclassified Desulfobacteraceae 251530, Balneola sp., Lactobacillus iners, Unclassified Bacteria 46553, Unclassified [Amoebophilaceae] 102524, Unclassified Phyllobacteriaceae 358715, Unclassified Bacteria 358716, Microbulbifer elongatus, Unclassified Flavobacteriaceae 358774, Unclassified Nitrospiraceae 9977, Unclassified Hyphomicrobiaceae 25362, Mycoplasma sp., Unclassified Lachnospiraceae 67609, Unclassified Hyphomicrobiaceae 106403, Unclassified Bacteria 155446, Unclassified Hyphomicrobiaceae 165088, Unclassified Alteromonadaceae 191057, Unclassified Rhodobacteraceae 220028, Unclassified Coxiellaceae 235007, Unclassified Colwelliaceae 358785, Unclassified Oleiphilaceae 358831, Unclassified Hyphomicrobiaceae 99435, Unclassified Bacteria 139823, Unclassified Oceanospirillaceae 181547, Unclassified Alteromonadaceae 209053, Unclassified Coxiellaceae 358916, Unclassified Coxiellaceae 358917, Unclassified Oleiphilaceae 358921, Unclassified Oleiphilaceae 358922, Unclassified Alteromonadaceae 358925
DNA analysis conducted by AquaBiomics LLC.