Certificate of Analysis: Live Reef Rubble, Batch LRR G10.3
Certificate of Analysis
lrr.g10.3_Certificate_of_Analysis
About this report
This report summarizes the results from DNA analysis of the community growing in and on a batch of Live Reef Rubble. Universal genetic markers were amplified from microbial (prokaryotic) and other (eukaryotic) DNA in the sample, and thousands of individual DNA molecules from this mixture were sequenced. Each sequence was then compared with public DNA databases to identify its origin. This report summarizes the kinds of organisms detected in the sample, with a focus on beneficial microbes, pathogens, and parasites.
Information about this sample
| Sample ID | lrr.g10.3 |
| Batch Name | G08-04-2025 |
| Sample Date | Sep 21, 2025 9:00 AM |
Microbial Diversity
This sample contained DNA from 388 different types of microbes.
This pie chart shows the diversity of each microbial family found in the sample. The size of each slice is proportional to the number of different types (labels on each slice). For clarity, only families containing 2 or more types are shown here. Unidentified sequences lacking any match in public databases are not shown.
Nitrifying Community
Ammonia-Oxidizing Microbes
| Type | Percent of Community | Genus | Species |
|---|---|---|---|
| 73 | 1.14 | Nitrosopumilus | Unknown |
| 108976 | 0.42 | Nitrosopumilus | Unknown |
| 9677 | 0.40 | Nitrosopumilus | Unknown |
| 31722 | 0.23 | Unknown | Unknown |
Nitrite-Oxidizing Bacteria
| Type | Percent of Community | Genus | Species |
|---|---|---|---|
| 318711 | 0.65 | Unknown | Unknown |
| 324314 | 0.02 | Unknown | Unknown |
The nitrifying community in this sample included 6 different types of microbes.
Bacterial Pathogens
None found.
Complete list of pathogens we screened for:
Eubacterium tarantellae, Lactococcus garvieae, Enterococcus seriolicida, Streptococcus parauberis, Streptococcus iniae, Mycobacterium chelonei, Mycobacterium fortuitum, Mycobacterium marinum, Mycobacterium neoaurum, Nocardia asteroides, Nocardia salmonicida, Nocardia seriolae, Renibacterium salmoninarum, Aeromonas jandaei, Aeromonas salmonicida, Serratia liquefaciens, Chryseobacterium balustinum, Chryseobacterium scophthalmum, Tenacibaculum maritimus, Tenacibaculum ovolyticus, Pasteurella skyensis, Pseudomonas anguilliseptica, Moritella marina, Moritella viscosa, Photobacterium damselae, Shewanella putrefaciens, Vibrio alginolyticus, Vibrio cholerae, Vibrio fischeri, Vibrio furnissii, Vibrio harveyi, Vibrio carchariae, Vibrio trachuri, Vibrio ichthyoenteri, Vibrio logei, Vibrio ordalii, Vibrio pelagius, Vibrio salmonicida, Vibrio splendidus, Halomonas cupida, Piscirickettsia salmonis, Vibrio shiloi, Vibrio coralliilyticus, Vibrio harveyi, Aurantimonas coralicida, Vibrio rotiferianus, Vibrio alginolyticus, Vibrio proteolyticus, Vibrio charcharvina, Serratia marcescens, Aquarickettsia rohweri
Eukaryotic Biodiversity
This sample contained DNA from 289 different Eukaryotic sources.
This pie chart shows the diversity of each Eukaryotic Phylum detected in the sample. The size of each slice is proportional to the number of different types from each phylum. For clarity, only families containing 2 or more types are shown here. Unidentified sequences lacking any match in public databases are not shown.
Parasites
None of the DNA sequences from this sample matched known parasites.
| Group | Description | Percent of eDNA |
|---|---|---|
| Amyloodinium | Dinoflagellate parasites responsible for the fish disease Marine Velvet | 0 |
| Cryptocaryon | Ciliate parasites responsible for the fish disease Ich | 0 |
| Brooklynella | Ciliate parasites responsible for the fish disease Brook | 0 |
| Uronema | Ciliate parasites responsible for a fish disease called by the same name | 0 |
| Phestilla | Montipora-eating Nudibranchs | 0 |
| Amakusaplana | Acropora-eating Flatworms | 0 |
| Ancyrocephalidae | Flatworms often called Flukes that parasitize fish | 0 |
| Gyrodactylidae | Flatworms often called Flukes that parasitize fish | 0 |
| Capsalidae | Flatworms often called Flukes that parasitize fish | 0 |
| Turbonilla | Snails in the family Pyramidellidae that parasitize giant clams | 0 |
| Trichodina | A ciliated protozoan parasite of fish | 0 |
Other Animal Pests
None of the DNA sequences from this sample matched known animal pests.
| Group | Description | Percent of eDNA |
|---|---|---|
| Aiptasia | The glass anemone, a ubiquitous pest | 0 |
| Thylacodes | Vermetid snails | 0 |
| Asterina | A group of sea stars that includes coral predators | 0 |
Complete list of Eukaryotes identified
This list includes all Eukaryotes (Animals, Protists, and anything else besides Bacteria or Archaea) that were detected in the sample and identified based on comparison with public DNA sequence databases. DNA sequences that lacked any matches in public datases could not be identified, and are not shown here. The list is sorted by abundance, from highest to lowest.
Glauconema trihymene (Ciliophora), Paracineta limbata (Ciliophora), Pseudovorticella paracratera (Ciliophora), Vaginicola crystallina (Ciliophora), Miamiensis avidus (Ciliophora), Psammodictyon constrictum (Diatomea), Navicula diserta (Diatomea), Rhaphoneis belgicae (Diatomea), Monosiga brevicollis (Eukaryota), Halothrix ambigua (Eukaryota), Choanoeca perplexa (Eukaryota), Scytosiphon lomentaria (Eukaryota), uncultured Corallinales sq21317 (Florideophycidae), uncultured Corallinales sq52775 (Florideophycidae), uncultured Corallinales sq328954 (Florideophycidae), Melobesioideae sp. (Florideophycidae), uncultured Corallinales sq141964 (Florideophycidae), uncultured Corallinales sq480227 (Florideophycidae), uncultured Corallinales sq480246 (Florideophycidae), Synarthrophyton patena (Florideophycidae), Gastroclonium pacificum (Florideophycidae), uncultured Corallinales sq496025 (Florideophycidae), Peyssonnelia novae-hollandiae (Florideophycidae), uncultured Corallinales sq482123 (Florideophycidae), Mastophoropsis canaliculata (Florideophycidae), uncultured Corallinales sq483215 (Florideophycidae), uncultured Corallinales sq483219 (Florideophycidae), uncultured Corallinales sq487209 (Florideophycidae), Mesophyllum lichenoides (Florideophycidae), Mesophyllum vancouveriense (Florideophycidae), uncultured Corallinales sq499158 (Florideophycidae), uncultured Corallinales sq495991 (Florideophycidae), uncultured Corallinales sq496042 (Florideophycidae), uncultured Corallinales sq502684 (Florideophycidae), uncultured Corallinales sq502718 (Florideophycidae), uncultured Corallinales sq502719 (Florideophycidae), uncultured Corallinales sq502720 (Florideophycidae), uncultured Corallinales sq502721 (Florideophycidae), uncultured Corallinales sq502722 (Florideophycidae), uncultured Corallinales sq502723 (Florideophycidae), uncultured Corallinales sq502724 (Florideophycidae), Chaetonotus sp. (Gastrotricha), Chaetonotus neptuni (Gastrotricha), uncultured eukaryote sq72 (Labyrinthulomycetes), Oblongichytrium sp. (Labyrinthulomycetes), uncultured marine sq2482 (Labyrinthulomycetes), uncultured eukaryote sq1120 (Labyrinthulomycetes), uncultured eukaryote sq269 (Labyrinthulomycetes), Thraustochytriidae sp. (Labyrinthulomycetes), uncultured eukaryote sq498118 (Labyrinthulomycetes), Aplanochytrium stocchinoi (Labyrinthulomycetes), Labyrinthuloides minuta (Labyrinthulomycetes), uncultured eukaryote sq496005 (Labyrinthulomycetes), uncultured eukaryote sq502678 (Labyrinthulomycetes), Aplanochytrium sp. (Labyrinthulomycetes), uncultured eukaryote sq502731 (Labyrinthulomycetes)
Complete List of Microbes Identified
This list includes all microbes (Bacteria & Archae) that were detected in the sample and identified based on comparison with public DNA sequence databases. DNA sequences that lacked any matches in public datases could not be identified, and are not shown here. The list is sorted by abundance, from highest to lowest.
Unclassified Desulfobacteraceae 122784, Unclassified Bacteria 46834, Unclassified Flavobacteriaceae 30384, Nitrosopumilus sp., Unclassified Flavobacteriaceae 318678, Unclassified Flavobacteriaceae 318679, Unclassified Flavobacteriaceae 318680, Unclassified Rhodobacteraceae 41, Unclassified Bacteria 112664, Unclassified Bacteria 106413, Unclassified Nitrospiraceae 318711, Unclassified Flavobacteriaceae 191510, Unclassified Bacteria 218750, Olleya sp., Unclassified Flavobacteriaceae 328, Unclassified Bacteria 1141, Unclassified Bacteria 40276, Unclassified Bacteria 949, Unclassified Bacteria 11774, Unclassified JdFBGBact 5336, Unclassified Phyllobacteriaceae 1255, Unclassified Piscirickettsiaceae 318778, Inquilinus sp., Unclassified Bacteria 2905, Unclassified Flammeovirgaceae 5837, Unclassified Bacteria 67570, Unclassified Bacteria 318840, Unclassified Verrucomicrobiaceae 71266, Unclassified Bacteria 35320, Muricauda sp., Glaciecola sp., Unclassified Hyphomicrobiaceae 9813, Unclassified Rhodobacteraceae 200155, Unclassified Saprospiraceae 1666, Unclassified Pirellulaceae 4596, Unclassified Oceanospirillaceae 13896, Unclassified NB1-i 319042, Unclassified Flavobacteriaceae 319043, Unclassified Piscirickettsiaceae 319044, Unclassified Flammeovirgaceae 9945, Unclassified Bacteria 209230, Unclassified Flavobacteriaceae 248662, Unclassified Piscirickettsiaceae 319099, HTCC sp., Unclassified Cenarchaeaceae 31722, Unclassified Flammeovirgaceae 12276, Fulvivirga sp., Unclassified Pirellulaceae 319291, Unclassified Flavobacteriaceae 319293, Unclassified Phycisphaeraceae 25388, Bacteriovorax sp., Unclassified Pirellulaceae 319412, Unclassified Bacteria 319416, Unclassified Thiotrichaceae 319417, Unclassified Flavobacteriaceae 3, Unclassified Bacteria 3025, Thalassomonas sp., Candidatus sp., Unclassified Flammeovirgaceae 109041, Unclassified Ellin6075 114540, Unclassified Bacteria 319590, Unclassified Bacteria 319593, Unclassified Bacteria 319596, Unclassified Flavobacteriaceae 319598, Unclassified Bacteria 668, Unclassified JdFBGBact 1704, Unclassified Bacteria 2783, Unclassified Clostridiaceae 5601, Unclassified Kordiimonadaceae 14565, Unclassified Xenococcaceae 319787, Unclassified Desulfobacteraceae 319789, Unclassified Desulfobacteraceae 319792, Arcobacter sp., Unclassified Bacteria 320084, Unclassified [Amoebophilaceae] 320087, Unclassified Bacteria 320089, Unclassified Bacteria 6932, Unclassified Bacteria 320449, Unclassified HTCC2089 320458, Unclassified Rhodobacteraceae 102, Unclassified Desulfobacteraceae 25546, Unclassified PAUC26f 71564, Mycobacterium sp., Unclassified Bacteria 320934, Unclassified Rhodothermaceae 320935, Unclassified Bacteria 320937, Unclassified Bacteria 193217, Unclassified Bacteria 321620, Unclassified Bacteria 321631, Unclassified Oceanospirillaceae 321634, Unclassified Saprospiraceae 321636, Unclassified Bacteria 321638, Unclassified Phyllobacteriaceae 9876, Corynebacterium sp., Unclassified Flammeovirgaceae 322639, Unclassified Bacteria 322648, Unclassified Bacteria 322649, Unclassified Bacteria 322661, Unclassified Rhodobacteraceae 322664, Unclassified Bacteria 322672, Unclassified Oceanospirillaceae 322679, Unclassified Bacteria 322689, Unclassified Bacteria 322690, Unclassified Desulfobacteraceae 322695, Unclassified Desulfobacteraceae 322701, Unclassified Bacteria 322704, Unclassified Bacteria 3692, Unclassified Bacteria 10830, Unclassified Bacteria 324307, Unclassified Bacteria 324309, Unclassified Phyllobacteriaceae 324311, Unclassified Bacteria 324313, Unclassified Nitrospiraceae 324314, Unclassified Xenococcaceae 324323, Unclassified Bacteria 324325, Unclassified Flammeovirgaceae 324326, Unclassified Bacteria 324329, Unclassified Bacteria 324330, Unclassified Rhodobacteraceae 324332, Unclassified Bacteria 324334, Unclassified Bacteria 324336, Unclassified Bacteria 324338, Unclassified Rhodobacteraceae 324340, Unclassified Rhodobacteraceae 324341, Unclassified Rhodobacteraceae 324342, Unclassified Bacteria 324344, Unclassified Bacteria 324348, Unclassified Thiotrichaceae 324354, Unclassified Flammeovirgaceae 324360, Spongiibacter sp., Unclassified Bacteria 324369, Unclassified Bacteria 324374, Unclassified Piscirickettsiaceae 324383, Unclassified Flammeovirgaceae 324388
DNA analysis conducted by AquaBiomics LLC.