Certificate of Analysis
lrr.ab57_Certificate_of_Analysis
About this report
This report summarizes the results from DNA analysis of the community growing in and on a batch of Live Reef Rubble. Universal genetic markers were amplified from microbial (prokaryotic) and other (eukaryotic) DNA in the sample, and thousands of individual DNA molecules from this mixture were sequenced. Each sequence was then compared with public DNA databases to identify its origin. This report summarizes the kinds of organisms detected in the sample, with a focus on beneficial microbes, pathogens, and parasites.
Information about this sample
| Sample ID | lrr.ab57 |
| Batch Name | lrr.ab57 |
| Sample Date | 07/29/26 08:20 AM |
Microbial Diversity
This sample contained DNA from 539 different types of microbes.
This graph shows the relative abundance of each Prokaryotic group in the DNA sample. Each slice represents a different Family, and the size of the slice is proportional to the fraction of DNA in the sample from that family. Unidentified sequences lacking any match in public databases are not shown. For clarity, only groups making up at least 0.5% of the sample are shown.
Nitrifying Community
Ammonia-Oxidizing Microbes
| Type | Percent of Community | Genus | Species |
|---|---|---|---|
| 2996 | 1.02 | Nitrosopumilus | Unknown |
| 73 | 0.73 | Nitrosopumilus | Unknown |
| 9677 | 0.65 | Nitrosopumilus | Unknown |
| 20 | 0.55 | Nitrosopumilus | Unknown |
| 112700 | 0.34 | Nitrosopumilus | Unknown |
Nitrite-Oxidizing Bacteria
| Type | Percent of Community | Genus | Species |
|---|---|---|---|
| 410564 | 0.1 | Unknown | Unknown |
The nitrifying community in this sample included 6 different types of microbes.
Bacterial Pathogens
None found.
Complete list of pathogens we screened for:
Eubacterium tarantellae, Lactococcus garvieae, Enterococcus seriolicida, Streptococcus parauberis, Streptococcus iniae, Mycobacterium chelonei, Mycobacterium fortuitum, Mycobacterium marinum, Mycobacterium neoaurum, Nocardia asteroides, Nocardia salmonicida, Nocardia seriolae, Renibacterium salmoninarum, Aeromonas jandaei, Aeromonas salmonicida, Serratia liquefaciens, Chryseobacterium balustinum, Chryseobacterium scophthalmum, Tenacibaculum maritimus, Tenacibaculum ovolyticus, Pasteurella skyensis, Pseudomonas anguilliseptica, Moritella marina, Moritella viscosa, Photobacterium damselae, Shewanella putrefaciens, Vibrio alginolyticus, Vibrio cholerae, Vibrio fischeri, Vibrio furnissii, Vibrio harveyi, Vibrio carchariae, Vibrio trachuri, Vibrio ichthyoenteri, Vibrio logei, Vibrio ordalii, Vibrio pelagius, Vibrio salmonicida, Vibrio splendidus, Halomonas cupida, Piscirickettsia salmonis, Vibrio shiloi, Vibrio coralliilyticus, Vibrio harveyi, Aurantimonas coralicida, Vibrio rotiferianus, Vibrio alginolyticus, Vibrio proteolyticus, Vibrio charcharvina, Serratia marcescens, Aquarickettsia rohweri
Eukaryotic Biodiversity
This sample contained DNA from 633 different Eukaryotic sources.
This pie chart shows the diversity of each Eukaryotic Phylum detected in the sample. The size of each slice is proportional to the number of different types from each phylum. For clarity, only families containing 2 or more types are shown here. Unidentified sequences lacking any match in public databases are not shown.
Parasites
None of the DNA sequences from this sample matched known parasites.
| Group | Description | Percent of eDNA |
|---|---|---|
| Amyloodinium | Dinoflagellate parasites responsible for the fish disease Marine Velvet | 0 |
| Cryptocaryon | Ciliate parasites responsible for the fish disease Ich | 0 |
| Brooklynella | Ciliate parasites responsible for the fish disease Brook | 0 |
| Uronema | Ciliate parasites responsible for a fish disease called by the same name | 0 |
| Phestilla | Montipora-eating Nudibranchs | 0 |
| Amakusaplana | Acropora-eating Flatworms | 0 |
| Ancyrocephalidae | Flatworms often called Flukes that parasitize fish | 0 |
| Gyrodactylidae | Flatworms often called Flukes that parasitize fish | 0 |
| Capsalidae | Flatworms often called Flukes that parasitize fish | 0 |
| Turbonilla | Snails in the family Pyramidellidae that parasitize giant clams | 0 |
| Trichodina | A ciliated protozoan parasite of fish | 0 |
Other Animal Pests
None of the DNA sequences from this sample matched known animal pests.
| Group | Description | Percent of eDNA |
|---|---|---|
| Aiptasia | The glass anemone, a ubiquitous pest | 0 |
| Thylacodes | Vermetid snails | 0 |
| Asterina | A group of sea stars that includes coral predators | 0 |
Complete list of Eukaryotes identified
This list includes all Eukaryotes (Animals, Protists, and anything else besides Bacteria or Archaea) that were detected in the sample and identified based on comparison with public DNA sequence databases. DNA sequences that lacked any matches in public datases could not be identified, and are not shown here. The list is sorted by abundance, from highest to lowest.
Chitinopoma serrula (Annelida), Novafabricia sp. (Annelida), Neodexiospira steueri (Annelida), Spirorbis spirorbis (Annelida), Trochonerilla mobilis (Annelida), Pileolaria sp. (Annelida), uncultured fungus 615456 (Aphelidea), uncultured fungus 617338 (Aphelidea), Multimonas koreensis (Apusomonadidae), Fabomonas tropica (Apusomonadidae), Ameira scotti (Arthropoda), Paramenophia sp. (Arthropoda), Zaus caeruleus (Arthropoda), Phyllothalestris sp. (Arthropoda), Culicoides variipennis (Arthropoda), Humulus lupulus (Ascomycota), Helotiales sp. (Ascomycota), Penicillium capsulatum (Ascomycota), Aspergillus cristatus (Ascomycota), Sclerotinia sclerotiorum (Ascomycota), Capronia pilosella (Ascomycota), Cladosporium sp. (Ascomycota), Placopsis gelida (Ascomycota), Bipolaris maydis (Ascomycota), Herpotrichia parasitica (Ascomycota), Graminopassalora graminis (Ascomycota), Debaryomyces udenii (Ascomycota), Erysiphe pisi (Ascomycota), Rhodotorula sp. (Basidiomycota), Sistotrema brinkmannii (Basidiomycota), Fomitopsis pinicola (Basidiomycota), uncultured fungus 3206 (Basidiomycota), Malassezia restricta (Basidiomycota), Filobasidium floriforme (Basidiomycota), uncultured fungus 615387 (Basidiomycota), Malassezia globosa (Basidiomycota), Cryptococcus sp. (Basidiomycota), Wallemia sebi (Basidiomycota), Holtermannia corniformis (Basidiomycota), Entoloma strictius (Basidiomycota), Dioszegia crocea (Basidiomycota), uncultured picobiliphyte 631 (Bicosoecida), uncultured eukaryote 56187 (Bicosoecida), Symbiomonas scintillans (Bicosoecida), uncultured eukaryote 603 (Bicosoecida), uncultured eukaryote 615482 (Bicosoecida), uncultured picobiliphyte 617390 (Bicosoecida), metagenome (Centrohelida), Paradinium poucheti (Cercozoa), Gymnophrys sp. (Cercozoa), Nuclearia-like filose (Cercozoa), Metopion fluens (Cercozoa), marine metagenome (Cercozoa), uncultured marine 66607 (Cercozoa), uncultured eukaryote 615500 (Cercozoa), Haplosporidian parasite (Cercozoa), uncultured eukaryote 345331 (Cercozoa), Protaspis sp. (Cercozoa), uncultured eukaryote 20918 (Cercozoa), Metromonas simplex (Cercozoa), uncultured freshwater 220400 (Cercozoa), Lotharella oceanica (Cercozoa), Lotharella globosa (Cercozoa), uncultured eukaryote 142146 (Cercozoa), uncultured Rhizaria 163170 (Cercozoa), uncultured eukaryote 278122 (Cercozoa), uncultured Oxytrichidae 615440 (Cercozoa), uncultured marine 617074 (Cercozoa), uncultured Chytridiomycota 71210 (Chytridiomycota), uncultured Chytridiomycota 34337 (Chytridiomycota), Chytridium polysiphoniae (Chytridiomycota), uncultured eukaryote 2287 (Ciliophora), Diophrys parappendiculata (Ciliophora), Cinetochilum ovale (Ciliophora), Prodiscocephalus borrori (Ciliophora), uncultured eukaryote 7447 (Ciliophora), Euplotes muscorum (Ciliophora), Uronychia transfuga (Ciliophora), Holosticha diademata (Ciliophora), Hemigastrostyla enigmatica (Ciliophora), Stichotrichia sp. (Ciliophora), Leptoamphisiella vermis (Ciliophora), Aspidisca hongkongensis (Ciliophora), Amphileptus litonotiformis (Ciliophora), Sathrophilus holtae (Ciliophora), Pseudourostyla cristata (Ciliophora), Strombidinopsis batos (Ciliophora), uncultured marine 4862 (Ciliophora), uncultured eukaryote 5040 (Ciliophora), Vaginicola sp. (Ciliophora), Euplotes euryhalinus (Ciliophora), Condylostentor auriculatus (Ciliophora), Psammodictyon constrictum (Diatomea), Sellaphora cf. (Diatomea), Amphora sp. (Diatomea), uncultured eukaryote 56 (Diatomea), uncultured marine 78751 (Diatomea), uncultured eukaryote 402670 (Diatomea), Nitzschia cf. (Diatomea), Cocconeis cf. (Diatomea), uncultured marine 480814 (Diatomea), Bacillariophyta sp. (Diatomea), Craticula molestiformis (Diatomea), Fragilaria capucina (Diatomea), uncultured eukaryote 308578 (Diatomea), Navicula vara (Diatomea), uncultured marine 44304 (Diatomea), Nanofrustulum shiloi (Diatomea), uncultured eukaryote 107562 (Diatomea), uncultured eukaryote 329705 (Diatomea), uncultured marine 14535 (Diatomea), Pleurosigma sp. (Diatomea), uncultured marine 510447 (Diatomea), uncultured marine 6976 (Diatomea), uncultured marine 26390 (Diatomea), Craticula pseudocitrus (Diatomea), Rossia sp. (Diatomea), uncultured marine 615631 (Diatomea), Nitzschia sigma (Diatomea), Psammodictyon panduriforme (Diatomea), uncultured marine 615888 (Diatomea), uncultured marine 354 (Diatomea), Licmophora gracilis (Diatomea), uncultured marine 206774 (Diatomea), uncultured marine 532989 (Diatomea), uncultured eukaryote 615750 (Diatomea), Bacillaria paxillifer (Diatomea), Cylindrotheca fusiformis (Diatomea), uncultured marine 616402 (Diatomea), uncultured marine 616405 (Diatomea), uncultured marine 616406 (Diatomea), uncultured marine 617003 (Diatomea), uncultured marine 617016 (Diatomea), uncultured marine 617077 (Diatomea), uncultured marine 617088 (Diatomea), uncultured eukaryote 617092 (Diatomea), uncultured marine 617330 (Diatomea), uncultured marine 617341 (Diatomea), uncultured marine 617374 (Diatomea), Symbiodinium microadriaticum (Dinoflagellata), Plagiodinium ballux (Dinoflagellata), Symbiodinium sp. (Dinoflagellata), uncultured eukaryote 5615 (Dinoflagellata), Testudodinium sp. (Dinoflagellata), Aduncodinium glandula (Dinoflagellata), Pfiesteria-like sp. (Dinoflagellata), Amphidinium sp. (Dinoflagellata), uncultured eukaryote 616055 (Dinoflagellata), Testudodinium maedaense (Dinoflagellata), Notosolenus urceolatus (Euglenozoa), Hemistasiidae sp. (Euglenozoa), Neobodo designis (Euglenozoa), uncultured eukaryote 71401 (Euglenozoa), Klosteria bodomorphis (Euglenozoa), uncultured kinetoplastid 616081 (Euglenozoa), uncultured kinetoplastid 403045 (Euglenozoa), uncultured kinetoplastid 615666 (Euglenozoa), uncultured eukaryote 615851 (Euglenozoa), Paraphysomonas imperforata (Eukaryota), Scytosiphon lomentaria (Eukaryota), Ectocarpus siliculosus (Eukaryota), Chattonella subsalsa (Eukaryota), Halothrix ambigua (Eukaryota), Hartmannella abertawensis (Eukaryota), Stygamoeba regulata (Eukaryota), Ministeria vibrans (Eukaryota), Telonema subtile (Eukaryota), uncultured eukaryote 615354 (Eukaryota), Ulvophyceae sp. (Eukaryota), Ciliophrys infusionum (Eukaryota), Ulvella viridis (Eukaryota), uncultured eukaryote 2861 (Eukaryota), eukaryote clone (Eukaryota), Goniomonas sp. (Eukaryota), uncultured eukaryote 680 (Eukaryota), uncultured eukaryote 1494 (Eukaryota), uncultured eukaryote 2829 (Eukaryota), uncultured marine 83364 (Eukaryota), Goniomonas aff. (Eukaryota), Cladophora socialis (Eukaryota), Goniomonas avonlea (Eukaryota), Acanthoeca spectabilis (Eukaryota), metagenome (Eukaryota), Prasinoderma coloniale (Eukaryota), uncultured marine 17626 (Eukaryota), Xanthophyceae sp. (Eukaryota), Picochlorum maculatum (Eukaryota), Coccoid pelagophyte (Eukaryota), uncultured eukaryote 3149 (Eukaryota), Sarcinochrysis marina (Eukaryota), Calliacantha sp. (Eukaryota), Paramoeba pemaquidensis (Eukaryota), Vannella sp. (Eukaryota), Paraflabellula hoguae (Eukaryota), Phaeophila dendroides (Eukaryota), Creolimax fragrantissima (Eukaryota), uncultured eukaryote 2515 (Eukaryota), uncultured eukaryote 3828 (Eukaryota), Paramoeba aestuarina (Eukaryota), Ochromonas sp. (Eukaryota), Pseudulvella consociata (Eukaryota), uncultured eukaryote 62580 (Eukaryota), uncultured eukaryote 281998 (Eukaryota), uncultured eukaryote 509151 (Eukaryota), uncultured eukaryote 612862 (Eukaryota), Lingulamoeba sp. (Eukaryota), uncultured eukaryote 615816 (Eukaryota), uncultured Dunaliellaceae 616041 (Eukaryota), Ochromonadaceae sp. (Eukaryota), uncultured eukaryote 616093 (Eukaryota), Tilopteris mertensii (Eukaryota), uncultured eukaryote 616311 (Eukaryota), Gloeotilopsis planctonica (Eukaryota), Lessonia variegata (Eukaryota), Monodus subterranea (Eukaryota), uncultured Corallinales 52775 (Florideophycidae), Ramicrusta textilis (Florideophycidae), uncultured Corallinales 616442 (Florideophycidae), Developayella elegans (Incertae_Sedis), Develorapax marinus (Incertae_Sedis), cf. Developayella (Incertae_Sedis), Schizochytrium minutum (Labyrinthulomycetes), Labyrinthuloides minuta (Labyrinthulomycetes), uncultured eukaryote 72 (Labyrinthulomycetes), Aplanochytrium kerguelense (Labyrinthulomycetes), Thraustochytrium sp. (Labyrinthulomycetes), Thraustochytriidae sp. (Labyrinthulomycetes), uncultured marine 1339 (Labyrinthulomycetes), Ulkenia profunda (Labyrinthulomycetes), Thraustochytrium multirudimentale (Labyrinthulomycetes), uncultured eukaryote 553493 (Labyrinthulomycetes), uncultured eukaryote 615382 (Labyrinthulomycetes), Oblongichytrium sp. (Labyrinthulomycetes), uncultured marine 574937 (Labyrinthulomycetes), uncultured eukaryote 615377 (Labyrinthulomycetes), Stellarchytrium dubum (Labyrinthulomycetes), uncultured eukaryote 273 (Labyrinthulomycetes), Aplanochytrium blankum (Labyrinthulomycetes), uncultured eukaryote 2090 (Labyrinthulomycetes), uncultured eukaryote 4861 (Labyrinthulomycetes), labyrinthulid quahog (Labyrinthulomycetes), uncultured stramenopile 21358 (Labyrinthulomycetes), uncultured eukaryote 2358 (Labyrinthulomycetes), uncultured marine 2482 (Labyrinthulomycetes), uncultured eukaryote 481868 (Labyrinthulomycetes), uncultured eukaryote 615589 (Labyrinthulomycetes), uncultured eukaryote 615517 (Labyrinthulomycetes), uncultured eukaryote 615907 (Labyrinthulomycetes), uncultured eukaryote 615968 (Labyrinthulomycetes), uncultured eukaryote 616223 (Labyrinthulomycetes), uncultured eukaryote 616322 (Labyrinthulomycetes), uncultured eukaryote 616415 (Labyrinthulomycetes), uncultured eukaryote 616423 (Labyrinthulomycetes), Labyrinthula sp. (Labyrinthulomycetes), uncultured eukaryote 617062 (Labyrinthulomycetes), uncultured eukaryote 617068 (Labyrinthulomycetes), uncultured eukaryote 617279 (Labyrinthulomycetes), uncultured eukaryote 617311 (Labyrinthulomycetes), uncultured eukaryote 617315 (Labyrinthulomycetes), uncultured eukaryote 617319 (Labyrinthulomycetes), uncultured eukaryote 617321 (Labyrinthulomycetes), Aplanochytrium stocchinoi (Labyrinthulomycetes), uncultured eukaryote 17616 (MAST-3), uncultured eukaryote 615590 (MAST-3), Camacolaimus sp. (Nematozoa), Monhysteridae environmental (Nematozoa), Achromadora cf (Nematozoa), Hordeum vulgare (Phragmoplastophyta), Allium cepa (Phragmoplastophyta), Quiina pteridophylla (Phragmoplastophyta)
Complete List of Microbes Identified
This list includes all microbes (Bacteria & Archae) that were detected in the sample and identified based on comparison with public DNA sequence databases. DNA sequences that lacked any matches in public datases could not be identified, and are not shown here. The list is sorted by abundance, from highest to lowest.
Unclassified Bacteria 791, Unclassified Rhodospirillaceae 346224, Unclassified Bacteria 27069, Unclassified Bacteria 2828, Unclassified Piscirickettsiaceae 334, Unclassified Bacteria 226201, Unclassified Oleiphilaceae 2870, Unclassified Rhodobacteraceae 11297, Nitrosopumilus sp., Unclassified Bacteria 16462, Unclassified Bacteria 159, Bacillus sp., Unclassified Bacteria 228342, Unclassified Rhodobacteraceae 276, Hyphomonas sp., Unclassified Bacteria 11399, Unclassified Bacteria 10020, Marinobacter sp., Unclassified Bacteria 74055, Unclassified Bacteria 11084, Mycobacterium sp., Unclassified Coxiellaceae 25916, Desulfococcus sp., Oleibacter sp., Unclassified Pirellulaceae 409980, Methylophaga sp., Unclassified Hyphomonadaceae 290, Unclassified Pirellulaceae 25441, Alteromonas sp., Oceanicaulis sp., Coraliomargarita sp., Unclassified koll13 61095, Unclassified Bacteria 224962, Unclassified Bacteria 409120, Unclassified Saprospiraceae 293, Microbulbifer sp., Unclassified Bacteria 12849, Unclassified Bacteria 431264, Candidatus sp., Unclassified Bacteria 431299, Alcanivorax sp., Vibrio sp., Amphritea sp., Unclassified Coxiellaceae 3520, Unclassified Hyphomicrobiaceae 410155, Unclassified wb1_P06 220550, Unclassified Hyphomicrobiaceae 410185, Unclassified Saprospiraceae 409, Unclassified Rhodothermaceae 3591, Unclassified Bacteria 10146, Unclassified Saprospiraceae 69158, A17 sp., Unclassified wb1_P06 431486, Unclassified Bacteria 431487, Unclassified OM60 431488, Unclassified Oleiphilaceae 431499, Unclassified Bacteria 1755, Unclassified Rhodospirillaceae 85963, Unclassified mitochondria 196497, Unclassified A4b 236485, Unclassified Cohaesibacteraceae 410256, Unclassified Bacteria 410257, Unclassified Piscirickettsiaceae 410258, Unclassified Bacteria 19751, Unclassified Bacteria 33870, Unclassified koll13 93630, Unclassified Hyphomicrobiaceae 955, Unclassified Bacteria 2597, Inquilinus sp., Unclassified Flavobacteriaceae 410456, Unclassified Rhodospirillaceae 431941, BD2-13 sp., Unclassified SC3-41 11933, Unclassified Saprospiraceae 64146, Unclassified Bacteria 88113, Unclassified Rhodospirillaceae 132612, Unclassified Nitrospiraceae 410564, Unclassified Erythrobacteraceae 432092, Unclassified Flammeovirgaceae 1479, Unclassified Piscirickettsiaceae 178417, Unclassified Pirellulaceae 410718, Unclassified Cohaesibacteraceae 410719, Unclassified Rhodospirillaceae 410720, Unclassified Bacteria 432321, Unclassified Bacteria 2816, Unclassified Ulvophyceae 5178, Unclassified Bacteria 6805, Unclassified Rhodospirillaceae 50288, Unclassified Rhodospirillaceae 432610, Unclassified Rhodobacteraceae 432611, Unclassified Bacteria 432640, Unclassified Bacteria 432644, Crocinitomix sp., Unclassified Bacteria 479, Unclassified Lentisphaeraceae 1218, Unclassified Cryomorphaceae 148918, Unclassified Cyanobacteriaceae 258835, Unclassified CV106 411200, Unclassified Bacteria 411202, Unclassified Piscirickettsiaceae 411203, Unclassified Bacteria 411204, Unclassified Verrucomicrobiaceae 433007, Unclassified Bacteria 433008, Unclassified JTB38 433049, Plesiocystis sp., Balneimonas sp., Unclassified Bacteria 377467, Unclassified Coxiellaceae 389194, Unclassified Cohaesibacteraceae 411646, Unclassified Bacteria 411647, Unclassified Bacteria 433550, Unclassified Flammeovirgaceae 433556, Unclassified PAUC26f 433557, Unclassified Pirellulaceae 433596, Unclassified Rhodobacteraceae 433606, Unclassified Flavobacteriaceae 6743, Unclassified Bacteria 116131, Unclassified Bacteria 129144, Unclassified Archaea 412532, Unclassified Phycisphaeraceae 412533, Unclassified Bacteria 412534, Unclassified Cohaesibacteraceae 412546, Unclassified Bacteria 412553, Unclassified Bacteria 434321, Unclassified Rhodobacteraceae 434324, Unclassified Pirellulaceae 434407, Unclassified Bacteria 434412, Unclassified Ulvophyceae 434419, Unclassified Bacteria 434421, Unclassified Bacteria 434422, Deinococcus sp., Unclassified Desulfovibrionaceae 434428, Unclassified Piscirickettsiaceae 9883, Anaerospora sp., Unclassified C111 262080, Unclassified Bacteria 414287, Unclassified Bacteria 414289, Unclassified Piscirickettsiaceae 414297, Unclassified Piscirickettsiaceae 414298, Unclassified Bacteria 414299, Unclassified Bacteria 414303, Unclassified Cohaesibacteraceae 414304, Unclassified Rhodospirillaceae 414305, Unclassified Piscirickettsiaceae 414308, Unclassified Piscirickettsiaceae 414309, Unclassified Piscirickettsiaceae 414313, Unclassified koll13 414316, Unclassified Saprospiraceae 422652, Unclassified Bacteria 435902, Unclassified Bacteria 435903, Unclassified Bacteria 435904, Unclassified Rhodospirillaceae 435905, Unclassified Rhodospirillaceae 435906, Unclassified Bacteria 435909, Unclassified Rhodospirillaceae 435910, Unclassified Bacteria 435912, Unclassified Bacteria 435913, Unclassified Rhodobacteraceae 435914, Unclassified Bacteria 435918, Unclassified Pirellulaceae 436085, Unclassified Coxiellaceae 436089, Unclassified Hyphomonadaceae 436094, Unclassified Bacteria 436096, Unclassified Saprospiraceae 436106, Unclassified Bacteria 436107, Unclassified Bacteria 436108, Unclassified Bacteria 436110, Unclassified Bacteria 436112, Unclassified Rhodobacteraceae 436113, Unclassified Rhodobacteraceae 436114, Unclassified Coxiellaceae 436122, Unclassified Oleiphilaceae 436123, Unclassified Oleiphilaceae 436128
DNA analysis conducted by AquaBiomics LLC.