Certificate of Analysis: Live Reef Rubble, Batch LRR G12.2
Certificate of Analysis
lrr.g12.2_Certificate_of_Analysis
About this report
This report summarizes the results from DNA analysis of the community growing in and on a batch of Live Reef Rubble. Universal genetic markers were amplified from microbial (prokaryotic) and other (eukaryotic) DNA in the sample, and thousands of individual DNA molecules from this mixture were sequenced. Each sequence was then compared with public DNA databases to identify its origin. This report summarizes the kinds of organisms detected in the sample, with a focus on beneficial microbes, pathogens, and parasites.
Information about this sample
| Sample ID | lrr.g12.2 |
| Batch Name | g04-17-2026 |
| Sample Date | 06/15/2026 09:20 AM |
Microbial Diversity
This sample contained DNA from 551 different types of microbes.
This pie chart shows the diversity of each microbial family found in the sample. The size of each slice is proportional to the number of different types (labels on each slice). For clarity, only families containing 2 or more types are shown here. Unidentified sequences lacking any match in public databases are not shown.
Nitrifying Community
Ammonia-Oxidizing Microbes
| Type | Percent of Community | Genus | Species |
|---|---|---|---|
| 108976 | 1.62 | Nitrosopumilus | Unknown |
| 2996 | 0.87 | Nitrosopumilus | Unknown |
| 73 | 0.83 | Nitrosopumilus | Unknown |
Nitrite-Oxidizing Bacteria
| Type | Percent of Community | Genus | Species |
|---|---|---|---|
| 10234 | 0.48 | Unknown | Unknown |
The nitrifying community in this sample included 4 different types of microbes.
Bacterial Pathogens
None found.
Complete list of pathogens we screened for:
Eubacterium tarantellae, Lactococcus garvieae, Enterococcus seriolicida, Streptococcus parauberis, Streptococcus iniae, Mycobacterium chelonei, Mycobacterium fortuitum, Mycobacterium marinum, Mycobacterium neoaurum, Nocardia asteroides, Nocardia salmonicida, Nocardia seriolae, Renibacterium salmoninarum, Aeromonas jandaei, Aeromonas salmonicida, Serratia liquefaciens, Chryseobacterium balustinum, Chryseobacterium scophthalmum, Tenacibaculum maritimus, Tenacibaculum ovolyticus, Pasteurella skyensis, Pseudomonas anguilliseptica, Moritella marina, Moritella viscosa, Photobacterium damselae, Shewanella putrefaciens, Vibrio alginolyticus, Vibrio cholerae, Vibrio fischeri, Vibrio furnissii, Vibrio harveyi, Vibrio carchariae, Vibrio trachuri, Vibrio ichthyoenteri, Vibrio logei, Vibrio ordalii, Vibrio pelagius, Vibrio salmonicida, Vibrio splendidus, Halomonas cupida, Piscirickettsia salmonis, Vibrio shiloi, Vibrio coralliilyticus, Vibrio harveyi, Aurantimonas coralicida, Vibrio rotiferianus, Vibrio alginolyticus, Vibrio proteolyticus, Vibrio charcharvina, Serratia marcescens, Aquarickettsia rohweri
Eukaryotic Biodiversity
This sample contained DNA from 1105 different Eukaryotic sources.
This pie chart shows the diversity of each Eukaryotic Phylum detected in the sample. The size of each slice is proportional to the number of different types from each phylum. For clarity, only families containing 2 or more types are shown here. Unidentified sequences lacking any match in public databases are not shown.
Parasites
None of the DNA sequences from this sample matched known parasites.
| Group | Description | Percent of eDNA |
|---|---|---|
| Amyloodinium | Dinoflagellate parasites responsible for the fish disease Marine Velvet | 0 |
| Cryptocaryon | Ciliate parasites responsible for the fish disease Ich | 0 |
| Brooklynella | Ciliate parasites responsible for the fish disease Brook | 0 |
| Uronema | Ciliate parasites responsible for a fish disease called by the same name | 0 |
| Phestilla | Montipora-eating Nudibranchs | 0 |
| Amakusaplana | Acropora-eating Flatworms | 0 |
| Ancyrocephalidae | Flatworms often called Flukes that parasitize fish | 0 |
| Gyrodactylidae | Flatworms often called Flukes that parasitize fish | 0 |
| Capsalidae | Flatworms often called Flukes that parasitize fish | 0 |
| Turbonilla | Snails in the family Pyramidellidae that parasitize giant clams | 0 |
| Trichodina | A ciliated protozoan parasite of fish | 0 |
Other Animal Pests
None of the DNA sequences from this sample matched known animal pests.
| Group | Description | Percent of eDNA |
|---|---|---|
| Aiptasia | The glass anemone, a ubiquitous pest | 0 |
| Thylacodes | Vermetid snails | 0 |
| Asterina | A group of sea stars that includes coral predators | 0 |
Complete list of Eukaryotes identified
This list includes all Eukaryotes (Animals, Protists, and anything else besides Bacteria or Archaea) that were detected in the sample and identified based on comparison with public DNA sequence databases. DNA sequences that lacked any matches in public datases could not be identified, and are not shown here. The list is sorted by abundance, from highest to lowest.
Planomonas micra (Ancyromonadida), uncultured marine 507 (Ancyromonadida), Chitinopoma serrula (Annelida), Parapionosyllis elegans (Annelida), Zaus caeruleus (Arthropoda), Euterpina acutifrons (Arthropoda), Limnohalacarus cultellatus (Arthropoda), uncultured marine 607704 (Arthropoda), uncultured marine 607747 (Arthropoda), Curvularia kusanoi (Ascomycota), Ophiosphaerella herpotricha (Ascomycota), Humulus lupulus (Ascomycota), Fusarium oxysporum (Ascomycota), Nanoscypha tetraspora (Ascomycota), Taphrina deformans (Ascomycota), Aureobasidium pullulans (Ascomycota), uncultured eukaryote 6838 (Ascomycota), Ramularia grevilleana (Ascomycota), Capronia pilosella (Ascomycota), Acremonium chrysogenum (Ascomycota), Debaryomyces udenii (Ascomycota), uncultured fungus 607714 (Ascomycota), Filobasidium floriforme (Basidiomycota), Malassezia globosa (Basidiomycota), Malassezia restricta (Basidiomycota), Itersonilia pannonica (Basidiomycota), Sporobolomyces roseus (Basidiomycota), Fomitopsis pinicola (Basidiomycota), Wallemia sebi (Basidiomycota), uncultured fungus 509 (Basidiomycota), Cryptococcus sp. (Basidiomycota), Lacrymaria velutina (Basidiomycota), Leucoagaricus barssii (Basidiomycota), Tremella leptogii (Basidiomycota), Exidia uvapassa (Basidiomycota), uncultured fungus 599877 (Basidiomycota), Richoniella asterospora (Basidiomycota), Melanopsichium pennsylvanicum (Basidiomycota), Pseudobodo tremulans (Bicosoecida), Bicosoeca vacillans (Bicosoecida), uncultured picobiliphyte 631 (Bicosoecida), uncultured eukaryote 599017 (Cercozoa), Gymnophrys sp. (Cercozoa), marine metagenome (Cercozoa), uncultured eukaryote 353420 (Cercozoa), uncultured marine 15485 (Cercozoa), uncultured marine 497451 (Cercozoa), uncultured eukaryote 599499 (Cercozoa), Paradinium poucheti (Cercozoa), Protaspis sp. (Cercozoa), Lotharella oceanica (Cercozoa), Esquamula lacrimiformis (Cercozoa), uncultured eukaryote 601317 (Cercozoa), uncultured eukaryote 599794 (Cercozoa), uncultured marine 607642 (Cercozoa), uncultured marine 607738 (Cercozoa), uncultured Chytridiomycota 1636 (Chytridiomycota), uncultured eukaryote 607730 (Chytridiomycota), Miamiensis avidus (Ciliophora), uncultured ciliate 21826 (Ciliophora), Cyclidium plouneouri (Ciliophora), Apobryophyllum schmidingeri (Ciliophora), uncultured Eimeriidae 607729 (Ciliophora), uncultured archaeon 599492 (Crenarchaeota), uncultured archaeon 84646 (Crenarchaeota), Psammodictyon constrictum (Diatomea), uncultured eukaryote 52878 (Diatomea), Craticula pseudocitrus (Diatomea), uncultured eukaryote 198850 (Diatomea), uncultured marine 532023 (Diatomea), uncultured marine 593085 (Diatomea), Pseudogomphonema sp. (Diatomea), Rossia sp. (Diatomea), uncultured eukaryote 6222 (Diatomea), Navicula arenaria (Diatomea), Amphora coffeiformis (Diatomea), uncultured stramenopile 267345 (Diatomea), uncultured marine 599478 (Diatomea), uncultured marine 4997 (Diatomea), Halamphora subtropica (Diatomea), Cocconeis cf. (Diatomea), Haslea spicula (Diatomea), uncultured eukaryote 56 (Diatomea), uncultured marine 6226 (Diatomea), uncultured marine 186955 (Diatomea), Chaetoceros sp. (Diatomea), Rhaphoneis sp. (Diatomea), uncultured marine 152476 (Diatomea), uncultured marine 600138 (Diatomea), Bacillariophyta sp. (Diatomea), Nitzschia communis (Diatomea), Cymbella cistuliformis (Diatomea), Navicula sp. (Diatomea), uncultured eukaryote 206394 (Diatomea), uncultured eukaryote 248209 (Diatomea), Licmophora juergensii (Diatomea), uncultured eukaryote 604665 (Diatomea), Haslea ostrearia (Diatomea), Entomoneis sp. (Diatomea), uncultured marine 17602 (Diatomea), uncultured marine 60613 (Diatomea), Amphora sp. (Diatomea), uncultured marine 302602 (Diatomea), uncultured marine 404229 (Diatomea), uncultured marine 406296 (Diatomea), Nitzschia cf. (Diatomea), uncultured eukaryote 544124 (Diatomea), uncultured eukaryote 545382 (Diatomea), uncultured marine 601060 (Diatomea), uncultured marine 601302 (Diatomea), Nitzschia microcephala (Diatomea), Pseudostaurosira elliptica (Diatomea), uncultured eukaryote 607685 (Diatomea), uncultured marine 607687 (Diatomea), uncultured marine 607688 (Diatomea), uncultured stramenopile 607689 (Diatomea), Bysmatrum arenicola (Dinoflagellata), Heterocapsa triquetra (Dinoflagellata), Leiocephalium pseudosanguineum (Dinoflagellata), uncultured eukaryote 206667 (Dinoflagellata), Symbiodinium sp. (Dinoflagellata), uncultured eukaryote 422 (Dinoflagellata), uncultured eukaryote 206378 (Dinoflagellata), Paragymnodinium shiwhaense (Dinoflagellata), Coolia canariensis (Dinoflagellata), uncultured marine 607678 (Dinoflagellata), uncultured eukaryote 607748 (Dinoflagellata), Rhynchopus sp. (Euglenozoa), Diplonema sp. (Euglenozoa), Neobodo designis (Euglenozoa), Bodonidae sp. (Euglenozoa), uncultured eukaryote 561213 (Euglenozoa), uncultured euglenozoan 599660 (Euglenozoa), Rhynchobodo sp. (Euglenozoa), uncultured eukaryote 600187 (Euglenozoa), uncultured eukaryote 601019 (Euglenozoa), Diplonema ambulator (Euglenozoa), uncultured kinetoplastid 604713 (Euglenozoa), Paraphysomonas imperforata (Eukaryota), Akkesiphycus lubricum (Eukaryota), Paramoeba eilhardi (Eukaryota), Paramoeba branchiphila (Eukaryota), Gloeotilopsis planctonica (Eukaryota), Vannella sp. (Eukaryota), Chattonella subsalsa (Eukaryota), Graesiella emersonii (Eukaryota), Ciliophrys infusionum (Eukaryota), Platyamoeba sp. (Eukaryota), Paramoeba aestuarina (Eukaryota), Coccomyxa subellipsoidea (Eukaryota), Ulvophyceae sp. (Eukaryota), uncultured eukaryote 35940 (Eukaryota), Halothrix ambigua (Eukaryota), metagenome (Eukaryota), uncultured eukaryote 600147 (Eukaryota), uncultured Nannochloris 600262 (Eukaryota), Chlorella sp. (Eukaryota), Pirum gemmata (Eukaryota), Picochlorum eukaryotum (Eukaryota), uncultured Nannochloris 601360 (Eukaryota), Vermistella antarctica (Eukaryota), Stygamoeba regulata (Eukaryota), Cladophora socialis (Eukaryota), Monosiga brevicollis (Eukaryota), Ulvella repens (Eukaryota), Symbiochloris sp. (Eukaryota), uncultured eukaryote 599029 (Eukaryota), Coccoid pelagophyte (Eukaryota), Psammamonas australis (Eukaryota), uncultured eukaryote 604663 (Eukaryota), uncultured Corallinales 52775 (Florideophycidae), Hildenbrandia occidentalis (Florideophycidae), Lithophyllum pustulatum (Florideophycidae), Pirsonia verrucosa (Incertae_Sedis), uncultured eukaryote 599819 (Incertae_Sedis), Schizochytrium minutum (Labyrinthulomycetes), Oblongichytrium sp. (Labyrinthulomycetes), unidentified (Labyrinthulomycetes), uncultured stramenopile 21358 (Labyrinthulomycetes), Thraustochytriidae sp. (Labyrinthulomycetes), uncultured eukaryote 72 (Labyrinthulomycetes), Thraustochytrium pachydermum (Labyrinthulomycetes), Ulkenia profunda (Labyrinthulomycetes), Thraustochytrium multirudimentale (Labyrinthulomycetes), Stellarchytrium dubum (Labyrinthulomycetes), uncultured marine 1339 (Labyrinthulomycetes), Labyrinthuloides minuta (Labyrinthulomycetes), uncultured eukaryote 1958 (Labyrinthulomycetes), Aplanochytrium kerguelense (Labyrinthulomycetes), uncultured eukaryote 1117 (Labyrinthulomycetes), Thraustochytrium sp. (Labyrinthulomycetes), uncultured marine 295175 (Labyrinthulomycetes), uncultured eukaryote 4861 (Labyrinthulomycetes), uncultured eukaryote 2458 (Labyrinthulomycetes), uncultured eukaryote 269 (Labyrinthulomycetes), uncultured eukaryote 1886 (Labyrinthulomycetes), uncultured marine 2482 (Labyrinthulomycetes), uncultured marine 136747 (Labyrinthulomycetes), Botryochytrium sp. (Labyrinthulomycetes), uncultured eukaryote 4942 (Labyrinthulomycetes), uncultured eukaryote 600362 (Labyrinthulomycetes), uncultured eukaryote 604705 (Labyrinthulomycetes), uncultured eukaryote 1233 (Labyrinthulomycetes), uncultured eukaryote 2131 (Labyrinthulomycetes), Aplanochytrium blankum (Labyrinthulomycetes), uncultured eukaryote 66634 (Labyrinthulomycetes), uncultured eukaryote 602764 (Labyrinthulomycetes), uncultured eukaryote 607647 (Labyrinthulomycetes), uncultured eukaryote 1352 (MAST-3), Stephanopyxis turris (MAST-3), uncultured eukaryote 406 (MAST-3), metagenome (Peronosporomycetes), Myzocytiopsis osiris (Peronosporomycetes), Haliphthoros sp. (Peronosporomycetes), Atkinsiella dubia (Peronosporomycetes), Halodaphnea panulirata (Peronosporomycetes), Festuca arundinacea (Phragmoplastophyta), Gossypium hirsutum (Phragmoplastophyta), Leuconia nivea (Porifera), Pericharax heteroraphis (Porifera), Sycon capricorn (Porifera), Grantiopsis heroni (Porifera), Aphroceras sp. (Porifera)
Complete List of Microbes Identified
This list includes all microbes (Bacteria & Archae) that were detected in the sample and identified based on comparison with public DNA sequence databases. DNA sequences that lacked any matches in public datases could not be identified, and are not shown here. The list is sorted by abundance, from highest to lowest.
Unclassified Hyphomicrobiaceae 1489, Curtobacterium sp., Unclassified Piscirickettsiaceae 139944, Unclassified Bacteria 16462, Unclassified Bacteria 1800, Nitrosopumilus sp., Unclassified Bacteria 36004, Unclassified Bacteria 791, Inquilinus sp., Unclassified Bacteria 9721, Unclassified Hyphomicrobiaceae 3790, Unclassified Hyphomicrobiaceae 9813, Unclassified Bacteria 30, Unclassified Bacteria 14517, Unclassified Colwelliaceae 717, Unclassified Rhodobacteraceae 67807, Desulfococcus sp., Marinobacter sp., Unclassified Bacteria 99482, Unclassified NB1-i 409904, Unclassified Bacteria 409905, Unclassified Bacteria 949, Unclassified Bacteria 108920, Microbulbifer sp., Xenococcus sp., Unclassified Nitrospiraceae 10234, Vibrio sp., Unclassified Bacteria 421570, Alteromonas sp., Unclassified Flammeovirgaceae 88338, Unclassified Bacteria 109162, Unclassified Bacteria 12806, Bacillus sp., Unclassified Flavobacteriaceae 26959, Unclassified Bacteria 1173, Unclassified Hyphomicrobiaceae 4662, Aquimarina sp., Unclassified Hyphomicrobiaceae 273, Unclassified Bacteria 62060, Unclassified Oleiphilaceae 689, Unclassified Xenococcaceae 43854, Unclassified Bacteria 132680, Unclassified Bacteria 218805, Unclassified Bacteria 1889, Alcanivorax sp., Unclassified Bacillaceae 4656, Unclassified Bacteria 359, Staphylococcus aureus, Unclassified Simkaniaceae 797, Microbacterium chocolatum, Unclassified Bacteria 10172, Unclassified Pirellulaceae 28028, Unclassified Bacteria 111159, Pseudoalteromonas sp., Photobacterium sp., Hyphomonas sp., Unclassified Oceanospirillaceae 1096, Unclassified Bacteria 2152, Unclassified Bacteria 10830, Oceanicaulis sp., Marinomonas sp., Unclassified Bacteria 219179, Unclassified Oceanospirillaceae 13896, Unclassified Colwelliaceae 61827, Unclassified Micrococcaceae 154917, Unclassified Lachnospiraceae 220739, Unclassified Hyphomicrobiaceae 364160, Roseivirga sp., Unclassified Bacteria 410212, Unclassified Ulvophyceae 410213, Unclassified Bacteria 1488, Unclassified Flammeovirgaceae 19043, Bacteriovorax sp., Unclassified Bacteria 114522, Unclassified Bacteria 7226, Unclassified Flavobacteriaceae 12147, Unclassified Bacteria 107030, Unclassified Phycisphaeraceae 141887, Unclassified Oceanospirillaceae 265739, Unclassified Bacteria 422343, Unclassified Pirellulaceae 977, Unclassified Rhodospirillaceae 1237, Unclassified Nocardioidaceae 60322, Brachybacterium sp., Unclassified Colwelliaceae 104640, Unclassified Bacteria 129144, Kaistobacter sp., Unclassified Colwelliaceae 258941, Unclassified Rhodospirillaceae 1122, Unclassified Hyphomonadaceae 3755, Arcobacter sp., Unclassified Bacteria 69236, Clostridium sp., Glaciecola sp., Unclassified Flammeovirgaceae 410471, Unclassified OM60 422680, Unclassified HTCC2089 422681, Unclassified Bacteria 1141, Unclassified Colwelliaceae 26532, Brevibacillus reuszeri, Unclassified Syntrophobacteraceae 40595, Unclassified Pirellulaceae 109572, Unclassified Bacteria 189623, Unclassified OM60 422916, Amphritea sp., Unclassified Oceanospirillaceae 55820, Skermanella sp., Unclassified Hyphomicrobiaceae 273175, Unclassified NB1-i 410750, LCP-26 sp., Unclassified Pirellulaceae 423207, Unclassified Bacteria 423211, Corynebacterium sp., Unclassified Saprospiraceae 1751, Propionibacterium acnes, Unclassified Pirellulaceae 26821, Unclassified Xenococcaceae 196104, Unclassified mitochondria 210746, Unclassified Bacteria 257403, Unclassified Piscirickettsiaceae 410948, Unclassified mb2424 423619, Unclassified Pseudonocardiaceae 423622, Unclassified Bacteria 3210, Unclassified Francisellaceae 3793, Unclassified Bacteria 33862, Lactobacillus iners, BD2-13 sp., Unclassified Flavobacteriaceae 122467, KSA1 sp., Unclassified Bacteria 411255, Unclassified Bacteria 411256, Unclassified Bacteria 411263, Unclassified Bacteria 159, Unclassified Ellin6075 38218, Unclassified Desulfobulbaceae 411726, Unclassified Flammeovirgaceae 411733, Planctomyces sp., Unclassified Pseudonocardiaceae 424971, Unclassified Bacteria 424972, Acinetobacter johnsonii, Unclassified Ellin6075 424988, Unclassified Microbacteriaceae 424989, Pseudonocardia sp., Enhydrobacter sp., Unclassified Bacteria 60726, Unclassified Desulfobacteraceae 122784, Unclassified koll13 132726, Unclassified Bacteriovoracaceae 197623, Unclassified Chitinophagaceae 207423, Unclassified Bacteria 377134, Unclassified Bacteria 392831, Unclassified Pirellulaceae 412745, Unclassified Hyphomicrobiaceae 412757, Unclassified Flavobacteriaceae 412761, Unclassified Bacteria 412762, Unclassified Xenococcaceae 412763, Unclassified Xenococcaceae 412764, Unclassified Bacteria 412765, Unclassified Piscirickettsiaceae 412772, Unclassified Bacteria 412773, Unclassified Piscirickettsiaceae 412777, Unclassified Piscirickettsiaceae 412778, Unclassified Bacteria 412782, Unclassified Oleiphilaceae 426409, Unclassified Hyphomicrobiaceae 426411, Unclassified Bacteria 426412, Unclassified Bacteria 426415, Unclassified Oceanospirillaceae 426417, Unclassified HTCC2089 426420, Unclassified Bacteria 426421, Unclassified Rhodospirillaceae 426424, Unclassified Bacteria 220749, Unclassified Oleiphilaceae 259332, Unclassified Bacteria 267773, Unclassified Legionellaceae 270953, Unclassified Bacteria 272165, Unclassified Bacteria 325573, Unclassified Hyphomicrobiaceae 371001, Unclassified Pirellulaceae 414611, Unclassified Pirellulaceae 414622, Unclassified Pirellulaceae 414623, Unclassified Hyphomicrobiaceae 414624, Unclassified Hyphomicrobiaceae 414628, Unclassified Piscirickettsiaceae 414629, Unclassified Bacteria 414630, Unclassified Flavobacteriaceae 414632, Unclassified Bacteria 414633, Unclassified Xenococcaceae 414635, Unclassified Xenococcaceae 414636, Unclassified Hyphomicrobiaceae 414641, Unclassified NB1-i 414642, Unclassified Bacteria 414645, Unclassified Bacteria 414651, Unclassified Bacteria 414652, Unclassified Bacteria 414653, Unclassified Pirellulaceae 414654, Unclassified Hyphomicrobiaceae 414656, Unclassified Hyphomicrobiaceae 414657, Unclassified Bacteria 414658, Unclassified Bacteria 414659, Unclassified Piscirickettsiaceae 414660, Marinobacter bryozoorum, Unclassified Piscirickettsiaceae 414666, Unclassified Piscirickettsiaceae 414667, Unclassified Piscirickettsiaceae 414668, Unclassified Hyphomicrobiaceae 414679, Unclassified Syntrophobacteraceae 414680, Unclassified Hyphomicrobiaceae 414685, Unclassified Bacteria 414691, Unclassified Bacteria 429023, Unclassified Bacteria 429025, Unclassified Pirellulaceae 429028, Unclassified Colwelliaceae 429036, Unclassified Bacteria 429039, Unclassified Rhodobacteraceae 429040, Unclassified Bacteria 429041, Unclassified Bacteria 429043, Unclassified Bacteria 429044, Unclassified Oceanospirillaceae 429047, Unclassified Colwelliaceae 429049, Unclassified Colwelliaceae 429050, Unclassified Legionellaceae 429051, Unclassified Bacteria 429055, Unclassified OM60 429057
DNA analysis conducted by AquaBiomics LLC.