Certificate of Analysis: Live Reef Rubble, Batch LRR G11.21
Certificate of Analysis
lrr.g11.21_Certificate_of_Analysis
About this report
This report summarizes the results from DNA analysis of the community growing in and on a batch of Live Reef Rubble. Universal genetic markers were amplified from microbial (prokaryotic) and other (eukaryotic) DNA in the sample, and thousands of individual DNA molecules from this mixture were sequenced. Each sequence was then compared with public DNA databases to identify its origin. This report summarizes the kinds of organisms detected in the sample, with a focus on beneficial microbes, pathogens, and parasites.
Information about this sample
| Sample ID | lrr.g11.21 |
| Batch Name | g12-15-25 |
| Sample Date | 03/20/2026 10:30 AM |
Microbial Diversity
This sample contained DNA from 487 different types of microbes.
This pie chart shows the diversity of each microbial family found in the sample. The size of each slice is proportional to the number of different types (labels on each slice). For clarity, only families containing 2 or more types are shown here. Unidentified sequences lacking any match in public databases are not shown.
Nitrifying Community
Ammonia-Oxidizing Microbes
| Type | Percent of Community | Genus | Species |
|---|---|---|---|
| 2503 | 0.08 | Unknown | Unknown |
| Type | Percent of Community | Genus | Species |
|---|---|---|---|
| 4288 | 0.39 | Candidatus | Unknown |
| Type | Percent of Community | Genus | Species |
|---|---|---|---|
| 73 | 1.72 | Nitrosopumilus | Unknown |
| 108976 | 1.41 | Nitrosopumilus | Unknown |
| 9677 | 1.16 | Nitrosopumilus | Unknown |
| 3535 | 1.06 | Cenarchaeum | symbiosum |
| 34770 | 0.41 | Unknown | Unknown |
Nitrite-Oxidizing Bacteria
| Type | Percent of Community | Genus | Species |
|---|---|---|---|
| 2912 | 1.74 | Unknown | Unknown |
The nitrifying community in this sample included 8 different types of microbes.
Bacterial Pathogens
None found.
Complete list of pathogens we screened for:
Eubacterium tarantellae, Lactococcus garvieae, Enterococcus seriolicida, Streptococcus parauberis, Streptococcus iniae, Mycobacterium chelonei, Mycobacterium fortuitum, Mycobacterium marinum, Mycobacterium neoaurum, Nocardia asteroides, Nocardia salmonicida, Nocardia seriolae, Renibacterium salmoninarum, Aeromonas jandaei, Aeromonas salmonicida, Serratia liquefaciens, Chryseobacterium balustinum, Chryseobacterium scophthalmum, Tenacibaculum maritimus, Tenacibaculum ovolyticus, Pasteurella skyensis, Pseudomonas anguilliseptica, Moritella marina, Moritella viscosa, Photobacterium damselae, Shewanella putrefaciens, Vibrio alginolyticus, Vibrio cholerae, Vibrio fischeri, Vibrio furnissii, Vibrio harveyi, Vibrio carchariae, Vibrio trachuri, Vibrio ichthyoenteri, Vibrio logei, Vibrio ordalii, Vibrio pelagius, Vibrio salmonicida, Vibrio splendidus, Halomonas cupida, Piscirickettsia salmonis, Vibrio shiloi, Vibrio coralliilyticus, Vibrio harveyi, Aurantimonas coralicida, Vibrio rotiferianus, Vibrio alginolyticus, Vibrio proteolyticus, Vibrio charcharvina, Serratia marcescens, Aquarickettsia rohweri
Eukaryotic Biodiversity
This sample contained DNA from 379 different Eukaryotic sources.
This pie chart shows the diversity of each Eukaryotic Phylum detected in the sample. The size of each slice is proportional to the number of different types from each phylum. For clarity, only families containing 2 or more types are shown here. Unidentified sequences lacking any match in public databases are not shown.
Parasites
None of the DNA sequences from this sample matched known parasites.
| Group | Description | Percent of eDNA |
|---|---|---|
| Amyloodinium | Dinoflagellate parasites responsible for the fish disease Marine Velvet | 0 |
| Cryptocaryon | Ciliate parasites responsible for the fish disease Ich | 0 |
| Brooklynella | Ciliate parasites responsible for the fish disease Brook | 0 |
| Uronema | Ciliate parasites responsible for a fish disease called by the same name | 0 |
| Phestilla | Montipora-eating Nudibranchs | 0 |
| Amakusaplana | Acropora-eating Flatworms | 0 |
| Ancyrocephalidae | Flatworms often called Flukes that parasitize fish | 0 |
| Gyrodactylidae | Flatworms often called Flukes that parasitize fish | 0 |
| Capsalidae | Flatworms often called Flukes that parasitize fish | 0 |
| Turbonilla | Snails in the family Pyramidellidae that parasitize giant clams | 0 |
| Trichodina | A ciliated protozoan parasite of fish | 0 |
Other Animal Pests
None of the DNA sequences from this sample matched known animal pests.
| Group | Description | Percent of eDNA |
|---|---|---|
| Aiptasia | The glass anemone, a ubiquitous pest | 0 |
| Thylacodes | Vermetid snails | 0 |
| Asterina | A group of sea stars that includes coral predators | 0 |
Complete list of Eukaryotes identified
This list includes all Eukaryotes (Animals, Protists, and anything else besides Bacteria or Archaea) that were detected in the sample and identified based on comparison with public DNA sequence databases. DNA sequences that lacked any matches in public datases could not be identified, and are not shown here. The list is sorted by abundance, from highest to lowest.
Terebella lapidaria (Annelida), Grassleia cf. (Annelida), Sabellastarte sp. (Annelida), Dinophilus gyrociliatus (Annelida), Polycirrus carolinensis (Annelida), Apomatus voightae (Annelida), uncultured eukaryote 567935 (Apicomplexa), uncultured eukaryote 568305 (Apicomplexa), Lankesteria hesperidiiformis (Apicomplexa), Artemia salina (Arthropoda), Mysis oculata (Arthropoda), Friedmanniomyces simplex (Ascomycota), Penicillium griseofulvum (Ascomycota), Cladosporium sp. (Ascomycota), Bagnisiella examinans (Ascomycota), Filobasidium floriforme (Basidiomycota), Malassezia restricta (Basidiomycota), Wallemia sebi (Basidiomycota), Stropharia ambigua (Basidiomycota), Neohygrocybe ingrata (Basidiomycota), uncultured marine 32863 (Cercozoa), Paradinium poucheti (Cercozoa), uncultured marine 15485 (Cercozoa), Haplosporidian parasite (Cercozoa), marine metagenome (Cercozoa), Philaster sinensis (Ciliophora), Dysteria pectinata (Ciliophora), Olindias phosphorica (Cnidaria), Eudendrium racemosum (Cnidaria), uncultured archaeon 569189 (Crenarchaeota), uncultured archaeon 83350 (Crenarchaeota), uncultured marine 141668 (Diatomea), Amphora sp. (Diatomea), Navicula reinhardtii (Diatomea), uncultured stramenopile 569751 (Diatomea), Pseudostaurosira elliptica (Diatomea), uncultured eukaryote 6222 (Diatomea), uncultured marine 6945 (Diatomea), Haslea ostrearia (Diatomea), uncultured eukaryote 574142 (Diatomea), Pfiesteria-like sp. (Dinoflagellata), Heterocapsa triquetra (Dinoflagellata), Symbiodinium sp. (Dinoflagellata), uncultured eukaryote 568724 (Euglenozoa), Diplonema sp. (Euglenozoa), Paraphysomonas imperforata (Eukaryota), Trebouxia asymmetrica (Eukaryota), uncultured marine 1201 (Eukaryota), uncultured eukaryote 568634 (Eukaryota), Rhinomonas pauca (Eukaryota), Scenedesmus sp. (Eukaryota), Ulvophyceae sp. (Eukaryota), Akkesiphycus lubricum (Eukaryota), Ulvella viridis (Eukaryota), Phaeophila dendroides (Eukaryota), uncultured stramenopile 573127 (Eukaryota), Scytosiphon lomentaria (Eukaryota), Peyssonnelia novae-hollandiae (Florideophycidae), Lithophyllum pustulatum (Florideophycidae), uncultured Corallinales 32406 (Florideophycidae), uncultured Corallinales 21317 (Florideophycidae), Peyssonnelia sp. (Florideophycidae), uncultured Corallinales 567854 (Florideophycidae), Peyssonnelia capensis (Florideophycidae), uncultured Corallinales 32421 (Florideophycidae), Renouxia sp. (Florideophycidae), Gastroclonium pacificum (Florideophycidae), uncultured Corallinales 325121 (Florideophycidae), uncultured Corallinales 32614 (Florideophycidae), uncultured Corallinales 52775 (Florideophycidae), uncultured Corallinales 207352 (Florideophycidae), uncultured Corallinales 337654 (Florideophycidae), uncultured Corallinales 568551 (Florideophycidae), uncultured Corallinales 152450 (Florideophycidae), uncultured Corallinales 569155 (Florideophycidae), uncultured Corallinales 142157 (Florideophycidae), uncultured Corallinales 325432 (Florideophycidae), uncultured Corallinales 326013 (Florideophycidae), uncultured Corallinales 64865 (Florideophycidae), Melobesioideae sp. (Florideophycidae), uncultured Corallinales 572506 (Florideophycidae), Synarthrophyton patena (Florideophycidae), uncultured Corallinales 2575 (Florideophycidae), uncultured Corallinales 141964 (Florideophycidae), uncultured Corallinales 147857 (Florideophycidae), uncultured Corallinales 199057 (Florideophycidae), uncultured Corallinales 403484 (Florideophycidae), uncultured Corallinales 404728 (Florideophycidae), uncultured Corallinales 422157 (Florideophycidae), Mesophyllum vancouveriense (Florideophycidae), uncultured Corallinales 568586 (Florideophycidae), uncultured Corallinales 569753 (Florideophycidae), Sonderopelta coriacea (Florideophycidae), Pachymenia cf. (Florideophycidae), uncultured Corallinales 574130 (Florideophycidae), Lithothamnion glaciale (Florideophycidae), uncultured Corallinales 574135 (Florideophycidae), uncultured Corallinales 574136 (Florideophycidae), uncultured Corallinales 574139 (Florideophycidae), uncultured eukaryote 72 (Labyrinthulomycetes), Aplanochytrium kerguelense (Labyrinthulomycetes), uncultured marine 2482 (Labyrinthulomycetes), uncultured eukaryote 518748 (Labyrinthulomycetes), Chaetopleura apiculata (Mollusca), Turbo cornutus (Mollusca), Spirastrella sp. (Porifera)
Complete List of Microbes Identified
This list includes all microbes (Bacteria & Archae) that were detected in the sample and identified based on comparison with public DNA sequence databases. DNA sequences that lacked any matches in public datases could not be identified, and are not shown here. The list is sorted by abundance, from highest to lowest.
Unclassified Desulfobacteraceae 122784, Unclassified Nitrospiraceae 2912, Nitrosopumilus sp., Unclassified Piscirickettsiaceae 55043, Unclassified Bacteria 5367, Inquilinus sp., Unclassified Piscirickettsiaceae 107004, Unclassified Bacteria 55095, Desulfococcus sp., Cenarchaeum symbiosum, Unclassified Hyphomicrobiaceae 1489, Unclassified Bacteria 218750, Unclassified Bacteria 1800, Unclassified Bradyrhizobiaceae 1033, Unclassified Bacteria 36004, Unclassified Bacteria 70287, Unclassified Piscirickettsiaceae 106987, Unclassified Pseudanabaenaceae 165660, Unclassified OM60 181898, Unclassified Bacteria 391000, Unclassified JdFBGBact 1704, Unclassified Pirellulaceae 391006, Unclassified Hyphomicrobiaceae 185751, Unclassified Rhodospirillaceae 55071, Unclassified Desulfobacteraceae 244808, Unclassified Pirellulaceae 391039, Unclassified Bacteria 16462, Unclassified Bacteria 67864, A17 sp., Unclassified Bacteria 391055, Unclassified Cenarchaeaceae 34770, Candidatus sp., Microbulbifer sp., Unclassified Clostridiaceae 292655, Unclassified Pirellulaceae 391088, Unclassified Flavobacteriaceae 30384, Clostridium sp., Unclassified Bacteria 201584, Unclassified Bacteria 391108, Unclassified Bacteria 235111, Alteromonas sp., Unclassified Bacteria 391170, Unclassified PAUC26f 391197, Thalassomonas sp., Unclassified Microbacteriaceae 241837, Unclassified Bacteria 334125, Mycobacterium sp., Unclassified Bacteria 391279, Unclassified Bacteria 5871, Unclassified Bacteria 126647, Unclassified Ulvophyceae 195264, Unclassified Peptostreptococcaceae 165872, Unclassified Coxiellaceae 66758, Planctomyces sp., Unclassified C111 15628, Unclassified Bacteria 391458, Unclassified Bacteria 9129, Unclassified Bacteria 9839, Unclassified Lachnospiraceae 220739, Unclassified Bacteria 245749, Unclassified Bacteria 391527, Unclassified Bacteria 391528, Unclassified Rhodobacteraceae 391530, Unclassified Verrucomicrobiaceae 35156, Unclassified Bacteria 63334, Staphylococcus aureus, Methylobacterium sp., Roseivirga sp., Unclassified Flammeovirgaceae 220481, Unclassified Bacteria 391720, Unclassified Syntrophobacteraceae 13230, Unclassified Saprospiraceae 67943, Unclassified Bacteria 172704, Unclassified Syntrophobacteraceae 194097, Unclassified Xenococcaceae 211113, Unclassified Desulfobacteraceae 368943, Unclassified Bacteria 391887, Unclassified Nitrosomonadaceae 2503, Unclassified Pirellulaceae 8175, Halomicronema sp., Unclassified Pseudanabaenaceae 250788, Unclassified Rhodospirillaceae 392092, Unclassified Piscirickettsiaceae 392095, Unclassified Flavobacteriaceae 392096, Unclassified Bacteria 392097, Unclassified Bacteria 359, Unclassified Bacillaceae 6949, Unclassified Piscirickettsiaceae 232152, Unclassified Piscirickettsiaceae 268846, Verrucomicrobium sp., Unclassified Rhodobacteraceae 392357, Unclassified Bacteria 392359, Unclassified Bacteria 392361, Unclassified Bacteria 392363, Unclassified Piscirickettsiaceae 392366, Unclassified Bacteria 392367, Bacillus sp., Unclassified Bacteria 392501, Phormidium sp., Unclassified Bacteria 1039, Unclassified Oceanospirillaceae 1193, Unclassified Bacteria 1889, Unclassified Pirellulaceae 19546, Unclassified Bacteria 60011, Balneola sp., Unclassified Bacteria 259418, Unclassified Bacteria 267572, Unclassified Pirellulaceae 392810, Unclassified Pseudanabaenaceae 392815, Unclassified Desulfobacteraceae 392826, Unclassified Bacteria 392830, Unclassified Bacteria 392831, Unclassified OM60 392833, Unclassified Bacteria 392834, Unclassified Flammeovirgaceae 392836, Unclassified Bacteria 392838, Unclassified PAUC26f 392839, Methylibium sp., Herbaspirillum sp., Unclassified Rhodospirillaceae 4370, SC3-56 sp., Unclassified Pirellulaceae 346334, Unclassified Desulfobacteraceae 392835, Unclassified C111 393610, Unclassified C111 393611, Unclassified Rhodospirillaceae 393615, Unclassified Bacteria 393618, Unclassified Bacteria 393625, Unclassified Hyphomicrobiaceae 393627, Unclassified Hyphomicrobiaceae 393634, Unclassified Bacteria 393635, Unclassified Piscirickettsiaceae 393637, Unclassified Rhodospirillaceae 393639, Unclassified Bacteria 393640, Unclassified Bacteria 393641, Unclassified Bacteria 393642, Unclassified Bacteria 393643, Unclassified Bacteria 393645, Unclassified Flammeovirgaceae 393653, Unclassified Bacteria 393658, Unclassified Desulfobacteraceae 393662, Unclassified Hyphomicrobiaceae 394074, Unclassified JdFBGBact 10625, Unclassified Piscirickettsiaceae 266086, Unclassified Flavobacteriaceae 366995, Unclassified Pirellulaceae 394923, Unclassified Piscirickettsiaceae 394924, Unclassified JdFBGBact 394928, Unclassified Pirellulaceae 394929, Unclassified OM60 394933, Unclassified Rhodospirillaceae 394937, Unclassified Rhodospirillaceae 394939, Unclassified Piscirickettsiaceae 394943, Unclassified Bacteria 394944, Unclassified Bacteria 394953, Unclassified Hyphomicrobiaceae 394954, Unclassified Bacteria 394955, Unclassified Bacteria 394961, Unclassified Pseudanabaenaceae 394963, Unclassified Hyphomicrobiaceae 394964, Unclassified Rhodospirillaceae 394967, Unclassified Bacteria 394971, Unclassified Bacteria 394974, Unclassified Bacteria 394975, Unclassified Bacteria 394976, Unclassified Bacteria 394977, Unclassified Bacteria 394979, Unclassified Bacteria 394980, Unclassified Bacteria 394981, Unclassified Bacteria 394982, Unclassified Bacteria 394983, Unclassified Bacteria 394984, Unclassified Bacteria 394987, Unclassified Bacteria 394991, Unclassified Rhodobacteraceae 394993, Unclassified Rhodospirillaceae 394994, Unclassified Rhodospirillaceae 394995, Unclassified Bacteria 394997, Unclassified Rhodobacteraceae 395001, Unclassified Flammeovirgaceae 395006, Unclassified Bacteria 395007, Unclassified Bacteria 395013, Unclassified Bacteria 395014, Unclassified Bacteria 395015, Unclassified Piscirickettsiaceae 395024, Unclassified Piscirickettsiaceae 395025, Unclassified Piscirickettsiaceae 395026, Unclassified Piscirickettsiaceae 395027, Unclassified Bacteria 395028, Unclassified Pseudanabaenaceae 395029, Unclassified Bacteria 395032, Unclassified Rhodospirillaceae 395033, Unclassified Pirellulaceae 395044, Unclassified Clostridiaceae 395046
DNA analysis conducted by AquaBiomics LLC.