1000511_tankDNA

About this report

Generated on: Thu Feb 24 16:32:49 2022

This report summarizes an analysis of eDNA (environmental DNA) in an aquarium water sample. Universal genetic markers were amplified from Eukaryotic DNA in the sample, and thousands of individual DNA molecules from this mixture were sequenced. Each sequence was then compared with public DNA databases to identify its origin. (You can read more about this method here).

This report summarizes the kinds of organisms detected in this sample, with a special focus on pathogens and parasites of concern in the saltwater aquarium industry and hobby.

Information about the sample

Sample ID	1000511
Sample Name	Test 2
Tank Name	180gal
Sample Date	2022-01-13 11:41 AM

Parasites

None of the DNA sequences from this sample matched known parasites.

View the full table

Group	Description	Percent of eDNA
Amyloodinium	Dinoflagellate parasites responsible for the fish disease Marine Velvet	0
Cryptocaryon	Ciliate parasites responsible for the fish disease Ich	0
Brooklynella	Ciliate parasites responsible for the fish disease Brook	0
Uronema	Ciliate parasites responsible for a fish disease called by the same name	0
Phestilla	Montipora-eating Nudibranchs	0
Amakusaplana	Acropora-eating Flatworms	0
Ancyrocephalidae	Flatworms often called Flukes that parasitize fish	0
Gyrodactylidae	Flatworms often called Flukes that parasitize fish	0
Capsalidae	Flatworms often called Flukes that parasitize fish	0
Turbonilla	Snails in the family Pyramidellidae that parasitize giant clams	0
Perkinsus	Apicomplexan parasites of clams and other bivalves	0
Trichodina	A ciliated protozoan parasite of fish	0

Dinoflagellates

Type	Percent of eDNA	Group	Name
sq1166	3.738	Alexandrium	Alexandrium insuetum
sq987	1.946	Ostreopsis	Ostreopsis cf.
sq239	0.307	Coolia	Coolia canariensis
sq34833	0.307	Alexandrium	Alexandrium insuetum
sq37241	0.051	Ostreopsis	Ostreopsis cf.

Most saltwater aquaria contain some Dinoflagellates, so the presence of this group should not be interpreted as a problem at low levels. High levels (>5% of the DNA, as a rule of thumb), may suggest a potential problem. Please note that Symbiodinium are included in this category but these are symbiotic, and do not pose any risk to the aquarium.

Diatoms

None of the DNA sequences from this sample matched Diatoms.

Eukaryotic Biodiversity

Diversity (Number of different types)	112
Number of Phyla	11
Number of Classes	17

This graph shows the diversity of each Eukaryotic group in the DNA sample. Each slice represents a different Class, and the size of the slice is proportional to the number of different types in that class. Unidentified sequences lacking any match in public databases are not shown.

Sample Composition

This graph shows the relative abundance of each Eukaryotic group in the DNA sample. Each slice represents a different Class, and the size of the slice is proportional to the fraction of DNA in the sample from that class. Unidentified sequences lacking any match in public databases are not shown.

Complete list of Eukaryotes identified

These tables, arranged by Class, list all Eukaryotic species that were detected in the sample and identified based on comparison with public DNA sequence databases. DNA sequences that lacked any matches in public datases could not be identified, and are not shown here. “Type” is an arbitrary number assigned to each unique sequence found in the database.

Complete Table

Anthozoa
Type	Percent of eDNA	Family	Name
sq5567	0.922	Scleractinia	Acropora aspera
sq15547	0.461	Scleractinia	Phyllangia mouchezii
sq34729	0.358	Scleractinia	Pavona varians
sq37239	0.051	Scleractinia	Astrangia poculata
sq37279	0.051	Scleractinia	Acropora aspera
Note:
Corals shown in this category may be misidentified since the marker used here does not have high resolution for identifying coral species.

Ascomycota
Type	Percent of eDNA	Family	Name
sq37260	0.051	Ascomycota	Kendrickiella phycomyces

Calcarea
Type	Percent of eDNA	Family	Name
sq53	1.843	Leucosolenida	Aphroceras sp.
sq34965	0.256	Leucosolenida	Aphroceras sp.
sq34322	0.102	Clathrinida	Leucetta sp.
sq37249	0.051	Leucosolenida	Aphroceras sp.
sq37271	0.051	Leucosolenida	Aphroceras sp.
sq37275	0.051	Leucosolenida	Aphroceras sp.

Chlorarachniophyta
Type	Percent of eDNA	Family	Name
sq1259	0.41	Chlorarachniophyta	Lotharella globosa

Corallinophycidae
Type	Percent of eDNA	Family	Name
sq1364	0.102	Corallinophycidae	Lithophyllum pustulatum
sq37273	0.051	Corallinophycidae	Hydrolithon cf.

Demospongiae
Type	Percent of eDNA	Family	Name
sq1470	1.587	Haplosclerida	Haliclona amboinensis
sq36017	0.102	Haplosclerida	Haliclona amboinensis
sq37261	0.051	Haplosclerida	Haliclona amboinensis
sq37277	0.051	Haplosclerida	Haliclona amboinensis

Dinophyceae
Type	Percent of eDNA	Family	Name
sq1166	3.738	Gonyaulacales	Alexandrium insuetum
sq987	1.946	Gonyaulacales	Ostreopsis cf.
sq239	0.307	Gonyaulacales	Coolia canariensis
sq34833	0.307	Gonyaulacales	Alexandrium insuetum
sq37241	0.051	Gonyaulacales	Ostreopsis cf.

Eukaryota
Type	Percent of eDNA	Family	Name
sq20	0.154	Cryptomonadales	Rhinomonas pauca
sq35424	0.154	Cryptomonadales	Rhinomonas pauca
sq37266	0.051	Cryptomonadales	Rhodomonas sp.

Eurotiomycetes
Type	Percent of eDNA	Family	Name
sq1052	0.563	Aspergillaceae	Aspergillus penicillioides
sq1135	0.307	Aspergillaceae	Aspergillus restrictus
sq35157	0.205	Aspergillaceae	Aspergillus caninus
sq2094	0.154	Aspergillaceae	Aspergillus penicillioides
sq37257	0.051	Aspergillaceae	Aspergillus neoflavipes
sq37258	0.051	Aspergillaceae	Aspergillus penicillioides

Gastropoda
Type	Percent of eDNA	Family	Name
sq34831	0.307	Vetigastropoda	Diodora graeca
sq35156	0.205	Caenogastropoda	Thylacodes adamsii
sq36013	0.102	Vetigastropoda	Diodora graeca
sq37250	0.051	Vetigastropoda	Diodora graeca

Hydrozoa
Type	Percent of eDNA	Family	Name
sq1478	0.358	Leptothecata	Clytia sp.

Intramacronucleata
Type	Percent of eDNA	Family	Name
sq17512	59.293	Licnophoria	Licnophora cf.
sq385	0.410	Oligohymenophorea	Metanophrys similis
sq34727	0.358	Oligohymenophorea	Pleuronema sp.
sq34832	0.307	Licnophoria	Licnophora cf.
sq35155	0.205	Licnophoria	Licnophora cf.
sq35419	0.154	Licnophoria	Licnophora cf.
sq37237	0.051	Licnophoria	Licnophora cf.

Labyrinthulomycetes
Type	Percent of eDNA	Family	Name
sq5059	0.717	Thraustochytriaceae	Thraustochytriidae sp.
sq35158	0.205	Labyrinthulomycetes	Stellarchytrium dubum
sq35426	0.154	Thraustochytriaceae	Thraustochytrium multirudimentale
sq35427	0.154	Thraustochytriaceae	Thraustochytriidae sp.
sq36018	0.102	Thraustochytriaceae	Thraustochytriidae sp.
sq37270	0.051	Thraustochytriaceae	Thraustochytriidae sp.

Phaeophyceae
Type	Percent of eDNA	Family	Name
sq128	4.199	Ectocarpales	Halothrix ambigua
sq124	1.075	Ectocarpales	uncultured eukaryote
sq36014	0.102	Ectocarpales	uncultured eukaryote
sq36015	0.102	Ectocarpales	uncultured eukaryote
sq37254	0.051	Phaeophyceae	Desmarestia viridis
sq37256	0.051	Phaeophyceae	Desmarestia viridis
sq37259	0.051	Ectocarpales	Tribonema marinum
sq37272	0.051	Ectocarpales	Tribonema marinum
sq37274	0.051	Ectocarpales	Tribonema marinum

Polychaeta
Type	Percent of eDNA	Family	Name
sq194	0.563	Sabellida	Rubifabriciola markginsbergi

Rhodymeniophycidae
Type	Percent of eDNA	Family	Name
sq36020	0.102	Rhodymeniophycidae	Peyssonnelia novae-hollandiae

Ulvophyceae
Type	Percent of eDNA	Family	Name
sq994	0.563	Ulvophyceae	Ulvella repens
sq34964	0.256	Ulvophyceae	Phaeophila dendroides

AquaBiomics tankDNA Test Report

1000511_tankDNA_Report