AquaBiomics tankDNA Test Report


About this report

Generated on: Fri Sep 23 09:14:32 2022

This report summarizes an analysis of eDNA (environmental DNA) in an aquarium water sample. Universal genetic markers were amplified from Eukaryotic DNA in the sample, and thousands of individual DNA molecules from this mixture were sequenced. Each sequence was then compared with public DNA databases to identify its origin. (You can read more about this method here).

This report summarizes the kinds of organisms detected in this sample, with a special focus on pathogens and parasites of concern in the saltwater aquarium industry and hobby.

Information about the sample

Sample ID 1000128
Sample Name Stock tank
Tank Name Stock tank
Sample Date 8/24/2022 17:45


Parasites

None of the DNA sequences from this sample matched known parasites.
View the full table
Group Description Percent of eDNA
Amyloodinium Dinoflagellate parasites responsible for the fish disease Marine Velvet 0
Cryptocaryon Ciliate parasites responsible for the fish disease Ich 0
Brooklynella Ciliate parasites responsible for the fish disease Brook 0
Uronema Ciliate parasites responsible for a fish disease called by the same name 0
Miamiensis Ciliate parasites responsible for a fish disease called Uronema or Scuticociliatosis 0
Phestilla Montipora-eating Nudibranchs 0
Amakusaplana Acropora-eating Flatworms 0
Prosthiostomum Acropora-eating Flatworms 0
Ancyrocephalidae Flatworms often called Flukes that parasitize fish 0
Gyrodactylidae Flatworms often called Flukes that parasitize fish 0
Capsalidae Flatworms often called Flukes that parasitize fish 0
Turbonilla Snails in the family Pyramidellidae that parasitize giant clams 0
Perkinsus Apicomplexan parasites of clams and other bivalves 0
Trichodina A ciliated protozoan parasite of fish 0


Dinoflagellates

Type Percent of eDNA Group Name
sq79092 0.233 Symbiodinium Symbiodinium sp.
sq80892 0.093 Polarella Polarella glacialis
sq80931 0.093 uncultured uncultured alveolate
sq80942 0.093 Halostylodinium Halostylodinium arenarium

Most saltwater aquaria contain some Dinoflagellates, so the presence of this group should not be interpreted as a problem at low levels. High levels (>5% of the DNA, as a rule of thumb), may suggest a potential problem. Please note that Symbiodinium are included in this category but these are symbiotic, and do not pose any risk to the aquarium.


Diatoms

Type Percent of eDNA Group Name
sq56 0.977 Cylindrotheca uncultured eukaryote
sq981 0.419 Entomoneis Entomoneis sp.
sq16393 0.419 Nitzschia Nitzschia cf. 
sq9 0.279 Fragilariales Convoluta convoluta
sq43342 0.140 Amphora Amphora cf. 
sq79803 0.140 Achnanthidium uncultured marine
sq6889 0.093 Fragilariales Convoluta convoluta
sq80926 0.093 Entomoneis Entomoneis sp.
sq80943 0.093 Nitzschia Nitzschia sigma
sq82422 0.047 Cylindrotheca uncultured eukaryote
sq82423 0.047 Entomoneis Entomoneis sp.
sq82483 0.047 Entomoneis uncultured eukaryote

Most saltwater aquaria contain some Diatoms, so the presence of this group should not be interpreted as a problem at low levels. High levels (>5% of the DNA, as a rule of thumb), may suggest a potential problem.


Eukaryotic Biodiversity

Diversity (Number of different types) 307
Number of Phyla 13
Number of Classes 18


This graph shows the diversity of each Eukaryotic group in the DNA sample. Each slice represents a different Class, and the size of the slice is proportional to the number of different types in that class. Unidentified sequences lacking any match in public databases are not shown.

Sample Composition


This graph shows the relative abundance of each Eukaryotic group in the DNA sample. Each slice represents a different Class, and the size of the slice is proportional to the fraction of DNA in the sample from that class. Unidentified sequences lacking any match in public databases are not shown.

Complete list of Eukaryotes identified

These tables, arranged by Class, list all Eukaryotic species that were detected in the sample and identified based on comparison with public DNA sequence databases. DNA sequences that lacked any matches in public datases could not be identified, and are not shown here. “Type” is an arbitrary number assigned to each unique sequence found in the database.

Complete Table
Actinopterygii
Type Percent of eDNA Family Name
sq79091 0.233 Actinopterygii Maccullochella peelii
sq13291 0.093 Actinopterygii Cyprinodon variegatus
Note:
This category includes all fish DNA in the sample, since the marker
used here shows little variation among fish species.
Anthozoa
Type Percent of eDNA Family Name
sq79829 0.140 Scleractinia Stylophora pistillata
sq17535 0.093 Anthozoa Clavularia sp.
sq80924 0.093 Scleractinia Stylophora pistillata
sq82415 0.047 Alcyonacea Xenia sp.
sq82470 0.047 Scleractinia Stephanocoenia intersepta
Note:
Corals shown in this category may be misidentified since the marker used here
does not have high resolution for identifying coral species.
Bacillariophyceae
Type Percent of eDNA Family Name
sq56 0.977 Bacillariophyceae uncultured eukaryote
sq981 0.419 Bacillariophyceae Entomoneis sp.
sq16393 0.419 Bacillariophyceae Nitzschia cf. 
sq43342 0.140 Bacillariophyceae Amphora cf. 
sq79803 0.140 Bacillariophyceae uncultured marine
sq80926 0.093 Bacillariophyceae Entomoneis sp.
sq80943 0.093 Bacillariophyceae Nitzschia sigma
sq82422 0.047 Bacillariophyceae uncultured eukaryote
sq82423 0.047 Bacillariophyceae Entomoneis sp.
sq82483 0.047 Bacillariophyceae uncultured eukaryote
Calcarea
Type Percent of eDNA Family Name
sq42 1.581 Leucosolenida Grantiopsis heroni
sq89 0.651 Leucosolenida Leucascandra caveolata
sq80891 0.093 Calcarea Eilhardia schulzei
sq80932 0.093 Leucosolenida Anamixilla sp.
sq80933 0.093 Leucosolenida Leucandra nicolae
sq80934 0.093 Leucosolenida Leucandra nicolae
sq80938 0.093 Leucosolenida Leucascandra caveolata
sq82411 0.047 Calcarea Eilhardia schulzei
sq82478 0.047 Leucosolenida Sycon ciliatum
sq82479 0.047 Leucosolenida Anamixilla sp.
sq82484 0.047 Leucosolenida Leucascandra caveolata
Choanoflagellida
Type Percent of eDNA Family Name
sq1879 1.116 Salpingoecidae Choanoeca perplexa
sq34370 0.093 Stephanoecidae uncultured eukaryote
sq82417 0.047 Salpingoecidae Choanoeca perplexa
sq82471 0.047 Salpingoecidae Choanoeca perplexa
sq82472 0.047 Salpingoecidae Choanoeca perplexa
Conoidasida
Type Percent of eDNA Family Name
sq79830 0.14 Eimeriorina Eimeria acervulina
Diatomea
Type Percent of eDNA Family Name
sq9 0.279 Fragilariales Convoluta convoluta
sq6889 0.093 Fragilariales Convoluta convoluta
Dinophyceae
Type Percent of eDNA Family Name
sq79092 0.233 Suessiaceae Symbiodinium sp.
sq80892 0.093 Suessiaceae Polarella glacialis
sq80931 0.093 uncultured uncultured alveolate
sq80942 0.093 Incertae_Sedis Halostylodinium arenarium
Diplonemea
Type Percent of eDNA Family Name
sq47331 0.186 Diplonemea Diplonema sp.
sq80884 0.093 Diplonemea Diplonema sp.
Euglenida
Type Percent of eDNA Family Name
sq79089 0.233 Heteronematina Notosolenus urceolatus
sq80883 0.093 Heteronematina Notosolenus urceolatus
Insecta
Type Percent of eDNA Family Name
sq79819 0.14 Hymenoptera Alysson spinosus
Intramacronucleata
Type Percent of eDNA Family Name
sq1936 1.349 Oligohymenophorea Cothurnia salina
sq79805 0.140 Oligohymenophorea Cothurnia salina
sq80887 0.093 Oligohymenophorea Cothurnia salina
sq80888 0.093 Oligohymenophorea Cothurnia salina
sq82491 0.047 Oligohymenophorea Vaginicola sp.
Kinetoplastea
Type Percent of eDNA Family Name
sq78982 0.279 Neobodonida uncultured eukaryote
Labyrinthulomycetes
Type Percent of eDNA Family Name
sq5925 0.884 Labyrinthulaceae Labyrinthula sp.
sq60756 0.186 Thraustochytriaceae uncultured marine
sq79304 0.186 Thraustochytriaceae uncultured marine
sq79306 0.186 Labyrinthulomycetes Stellarchytrium dubum
sq79828 0.140 Labyrinthulomycetes Stellarchytrium dubum
sq79832 0.140 Thraustochytriaceae uncultured marine
sq80920 0.093 Thraustochytriaceae Aplanochytrium kerguelense
sq80922 0.093 Thraustochytriaceae uncultured marine
sq80928 0.093 Labyrinthulomycetes Stellarchytrium dubum
sq80935 0.093 Labyrinthulomycetes Stellarchytrium dubum
sq80941 0.093 Labyrinthulomycetes Stellarchytrium dubum
sq82463 0.047 Thraustochytriaceae Thraustochytrium sp.
sq82465 0.047 Thraustochytriaceae Aplanochytrium sp.
Polychaeta
Type Percent of eDNA Family Name
sq214 0.465 Sabellida Spirorbis spirorbis
sq48 0.233 Palpata_Incertae_Sedis Trochonerilla mobilis
sq4157 0.140 Palpata_Incertae_Sedis Trochonerilla mobilis
sq4158 0.140 Palpata_Incertae_Sedis Paranerilla limicola
sq15956 0.140 Palpata_Incertae_Sedis Trochonerilla mobilis
sq79818 0.140 Palpata_Incertae_Sedis Trochonerilla mobilis
sq80905 0.093 Palpata_Incertae_Sedis Trochonerilla mobilis
sq76625 0.047 Sabellida Spirorbis spirorbis
sq82448 0.047 Palpata_Incertae_Sedis Trochonerilla mobilis
sq82454 0.047 Palpata_Incertae_Sedis Paranerilla limicola
Rhodymeniophycidae
Type Percent of eDNA Family Name
sq79834 0.14 Rhodymeniophycidae Peyssonnelia novae-hollandiae
Ulvophyceae
Type Percent of eDNA Family Name
sq341 0.186 Ulvophyceae Ulva sp.
sq79305 0.186 Ulvophyceae Ulva rigida
sq80929 0.093 Ulvophyceae Ulva rigida
sq82477 0.047 Ulvophyceae Ulva sp.
uncultured
Type Percent of eDNA Family Name
sq2132 0.186 uncultured uncultured eukaryote


DNA analysis conducted by AquaBiomics LLC.